LOCUS       sequence-152992-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged and C-terminal V5His6-tagged DmThor in S2 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Igreja C, Peter D, Weiler C, Izaurralde E
  TITLE     4E-BPs require non-canonical 4E-binding motifs and a lateral surface
            of eIF4E to repress translation.
  JOURNAL   Nat Commun. 2014 Sep 2;5:4790. doi: 10.1038/ncomms5790.
  PUBMED    25179781
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-09-2"; volume: "5:4790. doi"; pages: " 
            10.1038/ncomms5790"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmThor-V5His6
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..46
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(69..86)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(96..137)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(585..606)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     934..953
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atcgaggctg atcagcgagc tcaggcctta gaaggcacag tcgaggctga tcagcgggtt
       61 taaactcaat ggtgatggtg atgatgaccg gtacgcgtag aatcgagacc gaggagaggg
      121 ttagggatag gcttaccttc gaaccgcggg ccctctagac tcgagcgcag atccagttgg
      181 aactgttcct ggtcctcgat cttcagcgac ttggtctgct tgatcagttc cgtggggacg
      241 ggcacgcact tgcggaaggg agtacgcgga gtgcccctca gcaagcaact gggcacgttg
      301 gacggcggag tttggctcaa tggggagcca cggagattct tcatgaaagc ccgctcgtag
      361 ataagtttgg tgcctccagg agtggtggag tagagggttc cgccgggcgt cgaggagtac
      421 acctcgggca tctggatcgg atccgagatg acaaccttcc tggtgatcat gggcagggcc
      481 tgggtgatgg cttgacgggc ggtgggtgaa gcggacatga tatctgcaga attcaagctt
      541 ggcttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
      601 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag
      661 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
      721 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg
      781 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
      841 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
      901 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
      961 aagatggtgc gctcctg
//
