LOCUS       sequence-152994-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  More dimeric SE attached to Triple Mutant GEVI from Piao et al 2015.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Kang BE, Baker BJ
  TITLE     Pado, a fluorescent protein with proton channel activity can 
            optically monitor membrane potential, intracellular pH, and map gap 
            junctions.
  JOURNAL   Sci Rep. 2016 Apr 4;6:23865. doi: 10.1038/srep23865.
  PUBMED    27040905
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-04-4"; volume: "6:23865. doi"; pages: " 
            10.1038/srep23865"
COMMENT     Sequence Label: TM SE T206A
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      99..108
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(903..931)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgc caccatggag ggattcgacg
      121 gttcagattt tagtcctcca gctgatttag ttggcgttga cggtgcagtc atgcggaacg
      181 tcgttgacgt cacgataaat ggtgacgtca ctgctccgcc gaaagctgcg ccaagaaaaa
      241 gtgaatcggt aaagaaagtt cattggaatg atgtagacca aggaccgagt gaaaaaccag
      301 agacaagaca ggaggaacga atagatatac ccgagatttc aggtctatgg tggggcgaga
      361 atgaacatgg agtggacgat gggagaatgg agatacctac tactggtgta ggtcgcgtcc
      421 agtttcggat ccgagcagtg attgatcatc tagggatgcg agtctttgga gtcttcctaa
      481 ttttcttgga catcatcctc atgatcattg atctcagtct tatcgataaa agtgaatctt
      541 cacaatcctt ttatgacggg atggacttgg ctctttcttg ttatttcatg ctggatttag
      601 gattaaggat atttgcctac gggcccaagc ttttcttcac caacccttgg gaggtggctg
      661 atggtttgat tatcgttgtc acattcgtcg tcacgatatt ttacactgtg ttagatgaat
      721 acggtaccga aacaggagcc gatggtttgg ggcagttggt tgtgttggcc agattgctgc
      781 gtgtggttat cttagcaaga atattttatt cccaccaaca aatgaaggct tcaagcagaa
      841 gaacaatatc acagaacaaa agaagatatc gcatgagtaa aggagaagaa cttttcactg
      901 gagttgtccc aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca
      961 gtggagaggg tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta
     1021 ctggaaaact acctgttcca tggc
//
