LOCUS       sequence-152999-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used in the derivation of human iPS cells using non-integrating 
            episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA
  TITLE     Human Induced Pluripotent Stem Cells Free of Vector and Transgene 
            Sequences
  JOURNAL   Science. 2009 Mar 26.
  PUBMED    19325077
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Mar 26."
COMMENT     Sequence Label: pEP4 E02S EN2L
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(752..772)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 tttgcatatc tcctgaagat tttcattgtt gtcagcttcc tccacccact tctgcagcaa
       61 gggccgcagc ttacacatgt tcttgaagct aagctgcaga gcctcaaagc ggcagatggt
      121 cgtttggctg aataccttcc caaatagaac ccccagggtg agccccacat cggcctgtgt
      181 atatcccagg gtgatcctct tctgcttcag gagcttggca aattgctcga gttctttctg
      241 cagagctttg atgtcctggg actcctccgg gttttgctcc agcttctcct tctccagctt
      301 cacggcacca ggggtgacgg tgcagggctc cggggaggcc ccatcggagt tgctctccac
      361 cccgactcct gcttcaccct caggctgaga ggtctccaag ccgccttggg gcactagccc
      421 cactccaacc tggggcccac agtacgccat ccccccacag aactcatacg gcggggggca
      481 tgggggaatc ccccacacct cagagcctgg cccaaccccc ggcccgattc ctggccctcc
      541 aggagggcct tggaagctta gccaggtccg aggatcaacc cagcccggct ccggcccccc
      601 tggcccatca cctccaccac ctggaggggg cgagaaggcg aaatccgaag ccaggtgtcc
      661 cgccataatc actagttcga agcttgagct cgactgcaga attggccgcg accacgtgtt
      721 cacgacacct gaaatggaag aaaaaaactt tgaaccactg tctgaggctt gagaatgaac
      781 caagatccaa actcaaaaag ggcaaattcc aaggagaatt acatcaagtg ccaagctggc
      841 ctaacttcag tctccaccca ctcagtgtgg ggaaactcca tcgcataaaa cccctccccc
      901 caacctaaag acgacgtact ccaaaagctc cagaactaat cgaggtgcct ggacggcgcc
      961 cggtactccg tggagtcaca tgaagcgacg gctgaggacg gaaaggcccc tttcctttgt
     1021 gtggg
//
