LOCUS       sequence-153000-         996 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used in the derivation of human iPS cells using non-integrating 
            episomal vectors; expresses Oct4 and Sox2; Nanog and Lin28.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA
  TITLE     Human Induced Pluripotent Stem Cells Free of Vector and Transgene 
            Sequences
  JOURNAL   Science. 2009 Mar 26.
  PUBMED    19325077
REFERENCE   2  (bases 1 to 996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2009 Mar 26."
COMMENT     Sequence Label: pEP4 E02S EN2L
FEATURES             Location/Qualifiers
     source          1..996
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(649..666)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     876..895
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gacgcagaag gcctcagcac ctacctaccc cagcctttac tcttcctacc accagggatg
       61 cctggtgaac ccgactggga accttccaat gtggagcaac cagacctgga acaattcaac
      121 ctggagcaac cagacccaga acatccagtc ctggagcaac cactcctgga acactcagac
      181 ctggtgcacc caatcctgga acaatcaggc ctggaacagt cccttctata actgtggaga
      241 ggaatctctg cagtcctgca tgcagttcca gccaaattct cctgccagtg acttggaggc
      301 tgccttggaa gctgctgggg aaggccttaa tgtaatacag cagaccacta ggtattttag
      361 tactccacaa accatggatt tattcctaaa ctactccatg aacatgcaac ctgaagacgt
      421 gtgaagatga atcgaattcc cgcggccgct ttctcgaggc catcgatgtt taaacgatcc
      481 gcccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
      541 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
      601 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
      661 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
      721 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
      781 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
      841 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
      901 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
      961 acacatgctt tacatgtgtt tagtcgaggt taaaaa
//
