LOCUS       sequence-153019-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses proteins in the cytoplasm as fusions to maltose-binding 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Walker IH, Hsieh PC, Riggs PD
  TITLE     Mutations in maltose-binding protein that alter affinity and 
            solubility properties.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(1):187-97. doi: 
            10.1007/s00253-010-2696-y. Epub 2010 Jun 10.
  PUBMED    20535468
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2696-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "1"; pages: 
            "187-97"
COMMENT     Sequence Label: pMAL-c2E
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(157..162,163..178,179..185)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    193..209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     198..220
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 tgcttctggc gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac
       61 tgcataattc gtgtcgctca aggcgcactc ccgttctgga taatgttttt tgcgccgaca
      121 tcataacggt tctggcaaat attctgaaat gagctgttga caattaatca tcggctcgta
      181 taatgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagccag tccgtttagg
      241 tgttttcacg agcacttcac caacaaggac catagcatat gaaaatcgaa gaaggtaaac
      301 tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc ggtaagaaat
      361 tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg gaagagaaat
      421 tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca cacgaccgct
      481 ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg
      541 acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg attgcttacc
      601 cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg aacccgccaa
      661 aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt aagagcgcgc
      721 tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct gacgggggtt
      781 atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg gataacgctg
      841 gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac atgaatgcag
      901 acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg atgaccatca
      961 acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt gaattatggt gtaacggtac
     1021 tgccgacctt ca
//
