LOCUS       sequence-153020-        1036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses proteins in the cytoplasm as fusions to maltose-binding 
            protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Walker IH, Hsieh PC, Riggs PD
  TITLE     Mutations in maltose-binding protein that alter affinity and 
            solubility properties.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(1):187-97. doi: 
            10.1007/s00253-010-2696-y. Epub 2010 Jun 10.
  PUBMED    20535468
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2696-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "1"; pages: 
            "187-97"
COMMENT     Sequence Label: pMAL-c2E
FEATURES             Location/Qualifiers
     source          1..1036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     118..135
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     299..321
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     313..330
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     314..330
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(378..392)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(444..467)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg gtcaggtggg
       61 accaccgcgc tactgccgcc aggcaaattc tgttttatca gaccgcttct gcgttctgat
      121 ttaatctgta tcaggctgaa aatcttatct catccgccaa aacagccaag ctgccattcg
      181 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
      241 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
      301 cagtcacgac gttgtaaaac gacggccagt gccaagcttg cctgcaggtc gactctagag
      361 gatccgaatt ccggtacctt gtcatcgtca tccccgaggt tgttgttatt gttattgttg
      421 ttgttgttcg agctcgaatt agtctgcgcg tctttcaggg cttcatcgac agtctgacga
      481 ccgctggcgg cgttgatcac cgcagtacgc acggcatacc agaaagcgga catctgcggg
      541 atgttcggca tgatttcacc tttctgggcg ttttccatag tggcggcaat acgtggatct
      601 ttcgccaact cttcctcgta agacttcagc gctacggcac ccagcggttt gtctttatta
      661 accgcttcca gaccttcatc agtcagcaga tagttttcga ggaactcttt tgccagctct
      721 ttgttcggac tggcggcgtt aatacctgcg ctcagcacgc caacgaacgg tttggatggt
      781 tgacccttga aggtcggcag taccgttaca ccataattca ctttgctggt gtcgatgttg
      841 gaccatgccc acgggccgtt gatggtcatc gctgtttcgc ctttattaaa ggcagcttct
      901 gcgatggagt aatcggtgtc tgcattcatg tgtttgtttt taatcaggtc aaccaggaag
      961 gtcagacccg ctttcgcgcc agcgttatcc acgcccacgt ctttaatgtc gtacttgccg
     1021 ttttcatact tgaacg
//
