LOCUS       sequence-153032-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal tagging of human FANCF with 3xFLAG_Twin_Strep.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, 
            Doyon Y
  TITLE     A Scalable Genome-Editing-Based Approach for Mapping Multiprotein 
            Complexes in Human Cells.
  JOURNAL   Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009.
  PUBMED    26456817
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009."
COMMENT     Sequence Label: FancF_N_FS_v2_Donor
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      371..380
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             392..457
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(467..472,473..496,497..532,533..556)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
ORIGIN
        1 acctcaacac agattctagt tttaggcaaa gctcagaaaa tttctactta aggatatttc
       61 caaagcgaaa ggaagcgcgg agacgttcat gactggcatc atctcgcacg tggttccgga
      121 aattctcggt aggatgccct acatctgctc tccctccact aagaagaacc tctttgtgtg
      181 gcgaaagtaa aagtattagg gcttttaagt tgcccagagt caaggaacac ggataaagac
      241 gctgggagat tgacatgcat ttcgaccaat agcattgcag agaggcgtat catttcgcgg
      301 atgttccaat cagtacgcag agagtcgccg tctccaaggt gaaagcggaa gtagggcctt
      361 cgcgcacctc gccaccatgg gatccgccgg cgactacaag gaccacgacg gcgattataa
      421 ggatcacgac atcgactaca aagacgacga tgacaagggc gccagcagcg cctggtccca
      481 ccctcagttt gagaagggcg gaggctctgg cggcggaagc ggaggatctg cttggagcca
      541 cccccagttc gaaaagggcg ccgaatccct tctgcagcac ctggatcgct tttccgagct
      601 tctggcggtc tcaagcacta cctacgtcag cacctgggac cccgccaccg tgcgccgggc
      661 cttgcagtgg gcgcgctacc tgcgccacat ccatcggcgc tttggtcggc atggccccat
      721 tcgcacggct ctggagcggc ggctgcacaa ccagtggagg caagagggcg gctttgggcg
      781 gggtccagtt ccgggattag cgaacttcca ggccctcggt cactgtgacg tcctgctctc
      841 tctgcgcctg ctggagaacc gggccctcgg ggatgcagct cgttaccacc tggtgcagca
      901 actctttccc ggcccgggcg tccgggacgc cgatgaggag acactccaag agagcctggc
      961 ccgccttgcc cgccggcggt ctgcggtgca catgctgcgc ttcaatggct atagagaga
//
