LOCUS       sequence-153034-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal tagging of human EZH2 with 3xFLAG_Twin_Strep.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, 
            Doyon Y
  TITLE     A Scalable Genome-Editing-Based Approach for Mapping Multiprotein 
            Complexes in Human Cells.
  JOURNAL   Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009.
  PUBMED    26456817
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009."
COMMENT     Sequence Label: EZH2_N_FS_v2_Donor
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      404..413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             425..490
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             join(500..505,506..529,530..565,566..589)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
ORIGIN
        1 cattatgcct tgctactgta acagctaatg atgaaaaaca ggatctgtct ttatattttc
       61 ttccctccac aaatgtggat ctcatagagt tgaaaactag gttgtgatat agtatagtat
      121 acctaattcc tgtaatggga tcatgttcct ataatatggc cgcaatttag tgtagaattt
      181 ttgtaaataa aagtgtattt taagtttaac ttaaactttc aatgaagtgt tttaaggatt
      241 taaccatgca gcacaaatga gcacctttct gtaaatgcca acagtgtaat atgtgtcatt
      301 tcttcactga ttgttagttt gctgcggatt aaaacacagg tgatcatatt caggctggtt
      361 agattagtga ttttaatatg aaaccattgc ttttagaata atcgccacca tgggatccgc
      421 cggcgactac aaggaccacg acggcgatta taaggatcac gacatcgact acaaagacga
      481 cgatgacaag ggcgccagca gcgcctggtc ccaccctcag tttgagaagg gcggaggctc
      541 tggcggcgga agcggaggat ctgcttggag ccacccccag ttcgaaaagg gcgccggcca
      601 gactgggaag aaatctgaga agggaccagt ttgttggcgg aagcgtgtaa aatcagagta
      661 catgcgactg agacagctca agaggttcag acgagctgat gaagtaaagg tataatttta
      721 tcttttgtga aaatgaatat acaattaagg ataggttttt tcccctccta agttcaataa
      781 gtttttattt aaaataattc tagccaagtt tgtatttaca ataagatgag tgaatacttg
      841 gaagcttagg ttcttgatct gtcttaacat tataccatgc acattaatca gtctttgttt
      901 agtatacttt tatttgcata tgcagctgca gaaatgcctc cctaaatata aagggatgca
      961 tttcatgatt ttatgtttct aaagtcattg tgac
//
