LOCUS       sequence-153035-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  N-terminal tagging of human EZH2 with 3xFLAG_Twin_Strep.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, 
            Doyon Y
  TITLE     A Scalable Genome-Editing-Based Approach for Mapping Multiprotein 
            Complexes in Human Cells.
  JOURNAL   Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009.
  PUBMED    26456817
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009."
COMMENT     Sequence Label: EZH2_N_FS_v2_Donor
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(455..478,479..514,515..538,539..544))
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             complement(554..619)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      631..640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaccgcctct ccccgcgcgt tggccgattc attaatgcag aattctcgag tcacaatgac
       61 tttagaaaca taaaatcatg aaatgcatcc ctttatattt agggaggcat ttctgcagct
      121 gcatatgcaa ataaaagtat actaaacaaa gactgattaa tgtgcatggt ataatgttaa
      181 gacagatcaa gaacctaagc ttccaagtat tcactcatct tattgtaaat acaaacttgg
      241 ctagaattat tttaaataaa aacttattga acttaggagg ggaaaaaacc tatccttaat
      301 tgtatattca ttttcacaaa agataaaatt atacctttac ttcatcagct cgtctgaacc
      361 tcttgagctg tctcagtcgc atgtactctg attttacacg cttccgccaa caaactggtc
      421 ccttctcaga tttcttccca gtctggccgg cgcccttttc gaactggggg tggctccaag
      481 cagatcctcc gcttccgccg ccagagcctc cgcccttctc aaactgaggg tgggaccagg
      541 cgctgctggc gcccttgtca tcgtcgtctt tgtagtcgat gtcgtgatcc ttataatcgc
      601 cgtcgtggtc cttgtagtcg ccggcggatc ccatggtggc gattattcta aaagcaatgg
      661 tttcatatta aaatcactaa tctaaccagc ctgaatatga tcacctgtgt tttaatccgc
      721 agcaaactaa caatcagtga agaaatgaca catattacac tgttggcatt tacagaaagg
      781 tgctcatttg tgctgcatgg ttaaatcctt aaaacacttc attgaaagtt taagttaaac
      841 ttaaaataca cttttattta caaaaattct acactaaatt gcggccatat tataggaaca
      901 tgatcccatt acaggaatta ggtatactat actatatcac aacctagttt tcaactctat
      961 gagatccaca tttgtg
//
