LOCUS       sequence-153040-         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAGless construct expressing high specificity eSpCas9(1.1). 
            Px330-like plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, 
            Doyon Y
  TITLE     A Scalable Genome-Editing-Based Approach for Mapping Multiprotein 
            Complexes in Human Cells.
  JOURNAL   Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009.
  PUBMED    26456817
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009."
COMMENT     Sequence Label: eSpCas9(1.1)_No_FLAG
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..55)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 attcttactt tttctttttt gcctggccgg cctttttcgt ggccgccggc cttttgtcgc
       61 ctcccagctg agacaggtcg atccgtgtct cgtacaggcc ggtgatgctc tggtggatca
      121 gggtggcgtc cagcacctct ttggtgctgg tgtacctctt ccggtcgatg gtggtgtcaa
      181 agtacttgaa ggcggcaggg gctcccagat tggtcagggt aaacaggtgg atgatattct
      241 cggcctgctc tctgatgggc ttatcccggt gcttgttgta ggcggacagc actttgtcca
      301 gattagcgtc ggccaggatc actctcttgg agaactcgct gatctgctcg atgatctcgt
      361 ccaggtagtg cttgtgctgt tccacaaaca gctgtttctg ctcattatcc tcgggggagc
      421 ccttcagctt ctcatagtgg ctggccaggt acaggaagtt cacatatttg gagggcaggg
      481 ccagttcgtt tcccttctgc agttcgccgg cagaggccag cattctcttc cggccgtttt
      541 ccagctcgaa cagggagtac ttaggcagct tgatgatcag gtcctttttc acttctttgt
      601 agcccttggc ttccagaaag tcgatgggat tcttctcgaa gctgcttctt tccatgatgg
      661 tgatccccag cagctctttc acactcttca gtttcttgga cttgcccttt tccactttgg
      721 ccaccaccag cacagaatag gccacggtgg ggctgtcgaa gccgccgtac ttcttagggt
      781 cccagtcctt ctttctggcg atcagcttat cgctgttcct cttgggcagg atagactctt
      841 tgctgaagcc gcctgtctgc acctcggtct ttttcacgat attcacttgg ggcatgctca
      901 gcactttccg cacggtggca aaatcccggc ccttatccca cacgatctcc ccggtttcgc
      961 cgtttgtctc ga
//
