LOCUS       sequence-153041-         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Neuronal expression of GCaMP6f Ca sensor and physically separate 
            nuclear localized dTomato fluorophore .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  TITLE     Ting GCaMP6 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AAV-hSyn1-GCaMP6f-P2A-nls-dTomato
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             56..88
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(92..100,101..115)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      122..131
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             143..199
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     412..433
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(539..560)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 accggctaga ggatccggcg ccaccatggg ttctcatcat catcatcatc atggtatggc
       61 tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatct
      121 cgccaccatg gtcgactcat cacgtcgtaa gtggaataag acaggtcacg cagtcagagc
      181 tataggtcgg ctgagctcac tcgagaacgt ctatatcaag gccgacaagc agaagaacgg
      241 catcaaggcg aacttcaaga tccgccacaa catcgaggac ggcggcgtgc agctcgccta
      301 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
      361 cctgagcgtg cagtccaaac tttcgaaaga ccccaacgag aagcgcgatc acatggtcct
      421 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggcgg
      481 taccggaggg agcatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
      541 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggtgaggg
      601 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
      661 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
      721 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cc
//
