LOCUS       sequence-153045-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Untagged EZH2-TALEN_Left to target the N-terminus of human EZH2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Dalvai M, Loehr J, Jacquet K, Huard CC, Roques C, Herst P, Cote J, 
            Doyon Y
  TITLE     A Scalable Genome-Editing-Based Approach for Mapping Multiprotein 
            Complexes in Human Cells.
  JOURNAL   Cell Rep. 2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009.
  PUBMED    26456817
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep. 
            2015 Oct 7. pii: S2211-1247(15)01020-7. doi: 
            10.1016/j.celrep.2015.09.009."
COMMENT     Sequence Label: EZH2-TALEN_Left_No_FLAG
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..18)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(85..672)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 atggtgatgg tgatgatgac cggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaagggcc cttaaaagtt tatctcgccg ttattaaatt tccgtctgac
      121 ttcctctaag gttaatgtgc cggctttaat catttctcca ccaattaaaa gctcttctac
      181 actaagaaca gctccattac aattagtgat atgatttaat cgtgtaagct gagctttgta
      241 gtttccttta aagtgaccac tcacaaataa aaacttaaat tccgttacag aagatggata
      301 gactttccac cattcattag ggttgatatg tttgtttcgt gtttgatttt cttcgacata
      361 tcgttgcatt tcatctgctt ggccaattgg cagattataa cctccgctat aagctttagt
      421 atccacgatc acaccgtaat caataggaga tccgacagta taaattgctc cgtccggttt
      481 ccttgatcca cccaaatgtt tacctctata tccataaact ttcataaaaa attccattac
      541 cttcatttca agaattctat cctgagtgga atttctggca atttcaatta attcaatata
      601 ttcatgaggc acatatttca atttatgacg aagttcagat ttcttctcct ccagttcact
      661 tttgactagt tgggatcccg cgactcgatg ggaagttctc tcgggaatcc gccggttggt
      721 tcttttgatc aatgcgggag catgaggcag accctttttg actgcatcga gcgcgggtcg
      781 tccaccaaga catgccaacg ccaccagatg gtcattcgtt aacgcagcca acgcgggatc
      841 gggcctggac aattgggcta cgatggactc caaggctggc cgtcctcccc cattagaagc
      901 aatggcgacc acctgttcgg gtgtcagtcc atgatcctgg cacagtacag gcagcaggcg
      961 ctgtactg
//
