LOCUS       sequence-153047-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Saporin L3 in pichia.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Yuan H, Du Q, Sturm MB, Schramm VL
  TITLE     Soapwort Saporin L3 Expression in Yeast, Mutagenesis, and RNA 
            Substrate Specificity.
  JOURNAL   Biochemistry. 2015 Jul 28;54(29):4565-74. doi: 
            10.1021/acs.biochem.5b00405. Epub 2015 Jul 14.
  PUBMED    26091305
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.5b00405"; journalName: "Biochemistry"; date: 
            "2015-07-28- 28"; volume: "54"; issue: "29"; pages: "4565-74"
COMMENT     Sequence Label: pPICZalphaB-SapL3
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(41..415)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(434..481)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 atctccgagg cctcggaccc gtcgggccgc gtcggacgtg tcagtcctgc tcctcggcca
       61 cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct
      121 cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg
      181 accactcggc gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg
      241 gcaccacctg gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg
      301 cgaagtcgtc ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg
      361 ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt
      421 agttcctcac cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa
      481 caccgccctt agattagatt gctatgcttt ctttctaatg agcaagaagt aaaaaaagtt
      541 gtaatagaac aagaaaaatg aaactgaaac ttgagaaatt gaagaccgtt tattaactta
      601 aatatcaatg ggaggtcatc gaaagagaaa aaaatcaaaa aaaaaaattt tcaagaaaaa
      661 gaaacgtgat aaaaattttt attgcctttt tcgacgaaga aaaagaaacg aggcggtctc
      721 ttttttcttt tccaaacctt tagtacgggt aattaacgac accctagagg aagaaagagg
//
