LOCUS       sequence-153049-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in
            S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Zekri L, Kuzuoglu-Ozturk D, Izaurralde E
  TITLE     GW182 proteins cause PABP dissociation from silenced miRNA targets 
            in the absence of deadenylation.
  JOURNAL   EMBO J. 2013 Apr 3;32(7):1052-65. doi: 10.1038/emboj.2013.44. Epub 
            2013 Mar 5.
  PUBMED    23463101
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2013.44"; journalName: "EMBO J"; date: "2013-04-3- 
            3"; volume: "32"; issue: "7"; pages: "1052-65"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmeIF4G-V5His6
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..81
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             106..132
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gatcggggta ccaacatgga cgcacaaaca cgacgacgtg agcgtcgcgc tgagaaacaa
       61 gctcaatgga aagctgcaaa cggtggatct cctccacata tggcttaccc atacgatgtt
      121 ccagattacg ctcctccaag cttgaattct gcagatatcc agcacagtgg cggccgcatg
      181 caacaggcta taccaacttt accaacacaa tcagatatag ataaagccat gcagccccat
      241 tcagcacaaa atatgtatgt atcaacggga aacaacaatt ctgggaatac ccgttctaat
      301 ccacaaagtg gcggaatatt tcgaggacca ccctcgacgc ctaatgcccc tagaggtgct
      361 agtggcggag caacacggca tgtccatgtg caacccatgt actcacaacc ccttcaccag
      421 aacatggtgc tacaacaata cacacaatat aacccacggc aacaaacatt tccagcttct
      481 catttgcaat acgcacccgc ccccatgcca tactaccagt accaatatgt cccaactctt
      541 cagcaacagc cgccgcatac tcgtagtgct gtcacggtaa atacgaacgt caatgtggga
      601 aacaatttgc agccagtgca gtctggaccc aatggacctc tccctgttcc gggggctagc
      661 tcgtcgcaaa ttcaattgct cacaagcaca gtgcagccag gagccagcac tgtaatgggc
      721 gttggcggtc ctggtagtac aatggggcaa gtgggtgtgc caccaatggt tggggttggt
      781 gtaatgacaa caagtgtgca accgcaacct gtgcaggtgc agcctgctag ccggcgccgg
      841 catcagcatc gattgcagat aatcgatccg acgactaaaa aaaatatttt agacgatttt
      901 gataagacaa aatcaaacac agacaatgaa ttttccgatc aggtaacctc cacaaacaca
      961 ccagcaactg tcctttcaga aggtccaata cgtattccac agcaagaaag tgttgg
//
