LOCUS       sequence-153050-         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses lambdaN-HA-tagged and C-terminal V5-His6-tagged DmeIF4G in
            S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Zekri L, Kuzuoglu-Ozturk D, Izaurralde E
  TITLE     GW182 proteins cause PABP dissociation from silenced miRNA targets 
            in the absence of deadenylation.
  JOURNAL   EMBO J. 2013 Apr 3;32(7):1052-65. doi: 10.1038/emboj.2013.44. Epub 
            2013 Mar 5.
  PUBMED    23463101
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2013.44"; journalName: "EMBO J"; date: "2013-04-3- 
            3"; volume: "32"; issue: "7"; pages: "1052-65"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmeIF4G-V5His6
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..55
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(78..95)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(105..146)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ggatcctcga tcgaggctga tcagcgagct caggccttag aaggcacagt cgaggctgat
       61 cagcgggttt aaactcaatg gtgatggtga tgatgaccgg tacgcgtaga atcgagaccg
      121 aggagagggt tagggatagg cttaccttcg aaccgcgggc cctctagagg gttggcatca
      181 tcgtttaaca acgagttaaa aaaaggatta agacttttta cagcaacgcc cttgccagca
      241 gactgatcct ttgaatctcg ccacttacaa agcgattcct tctgtattac aaaggcatca
      301 tatagctcgc caattagttc actcagcaac cctcttggat gctccaatcc atgaactaaa
      361 agctgcaaag tatataaaca ttcaagttgg tggtcttcat tagaatcaat gtatctctgc
      421 aaaaccggta tgcaaaactt ttgaaaggtt tcggactcaa gcttgtaact gttgtccttg
      481 tagtgaatcg agaaattact caatgtttcc gtcaatcctc tgataaacaa cttatcaacc
      541 accattatat ttccgttact ataatcaata atgcagtccg ccgttgttcc ttcctttagt
      601 aagtgctcaa tatggtctat cacattttta acgtgctttt ctggagtctc tctatgatca
      661 atcactggcg actttgattc gttttccaca tattccagtc tgttaaattt aataaagtcg
      721 gcaacttcac tttcaggcat aaaatctgac cattttaggt taaacttgat ccacatattc
      781 cgagcaaaat ttggtccaac ttcttgtgtg caatatatca agtatgtttt aaggaacttt
      841 ttagcgacgt ttgatggact attttctttt aaattgttgt tccacaaatc tgatatagtc
      901 aatattttct ttacaattag tggcccggca aattgaagaa tgtatagcca aagctccgga
      961 atatcaacaa tcaaatcgtt tg
//
