LOCUS       sequence-153051-        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Hogg M, Seki M, Wood RD, Doublie S, Wallace SS
  TITLE     Lesion bypass activity of DNA polymerase theta (POLQ) is an 
            intrinsic property of the pol domain and depends on unique sequence 
            inserts.
  JOURNAL   J Mol Biol. 2011 Jan 21;405(3):642-52. doi: 
            10.1016/j.jmb.2010.10.041. Epub 2010 Nov 2.
  PUBMED    21050863
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2010.10.041"; journalName: "J Mol Biol"; date: 
            "2011-01-21- 21"; volume: "405"; issue: "3"; pages: "642-52"
COMMENT     Sequence Label: Sumo3 PolQM1 deltains3
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             77..346
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 3"
                     /label=SUMO3
                     /translation="EEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYC
                     ERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggtcatc accatcatca
       61 tcacgggtcc ctgcaggagg agaagcccaa ggagggtgtg aagacagaga atgaccacat
      121 caacctgaag gtggccgggc aggacggctc cgtggtgcag ttcaagatca agaggcacac
      181 gccgctgagc aagctgatga aggcctactg cgagaggcag ggcttgtcaa tgaggcagat
      241 cagattcagg ttcgacgggc agccaatcaa tgaaactgac actccagcac agctggagat
      301 ggaggacgag gacaccatcg acgtgttcca gcagcagacg ggaggtgggt tcaaagacaa
      361 cagccctatt agtgacacaa gcttttcact tcagttatca caggatggat tacagttaac
      421 tccagcctca agcagttcag aaagtttgtc cataattgat gtagcaagtg accaaaatct
      481 tttccaaaca ttcattaagg agtggcggtg caaaaagcga ttttccatct cactggcttg
      541 tgaaaagatt agaagtttga catcttctaa aactgctact attggcagta ggtttaagca
      601 agctagctca cctcaggaaa ttcctattag agatgatgga tttcccatta aaggttgtga
      661 tgacaccttg gtggttggac tggcagtatg ctggggtgga agggatgcct attatttttc
      721 actgcagaag gaacaaaagc attctgaaat tagtgccagt ttggttccac cttctttaga
      781 tccaagcctg actttgaaag acaggatgtg gtaccttcaa tcttgcttgc gaaaggaatc
      841 tgataaagaa tgttctgttg tcatctatga cttcatccag agctataaaa ttcttcttct
      901 ttcttgtggc atctccttgg agcaaagtta tgaagatcct aaggtggcat gctggttact
      961 agatccagat tctcaggagc cgactcttca tagcatagtt ac
//
