LOCUS       sequence-153053-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged DmeIF4E in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Zekri L, Kuzuoglu-Ozturk D, Izaurralde E
  TITLE     GW182 proteins cause PABP dissociation from silenced miRNA targets 
            in the absence of deadenylation.
  JOURNAL   EMBO J. 2013 Apr 3;32(7):1052-65. doi: 10.1038/emboj.2013.44. Epub 
            2013 Mar 5.
  PUBMED    23463101
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2013.44"; journalName: "EMBO J"; date: "2013-04-3- 
            3"; volume: "32"; issue: "7"; pages: "1052-65"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmeIF4E
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             814..855
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             865..882
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(905..922)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(979..998)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gacgagctgt acaagccaag cttgaattct atggtagtgt tggagacgga gaagaccagc
       61 gcccccagca ccgagcaggg tcgtccggaa ccaccaactt cggctgcagc gcccgccgag
      121 gctaaggatg tcaagcccaa ggaggaccca caggagactg gtgaaccagc aggcaacact
      181 gcaaccacta ctgctcctgc cggcgacgat gctgtgcgca ccgagcattt atacaaacac
      241 ccgctcatga atgtctggac gctgtggtac cttgaaaacg atcggtccaa gtcctgggag
      301 gacatgcaaa acgagatcac cagcttcgat accgtcgagg acttctggag cctatacaac
      361 cacatcaagc ccccatcaga gatcaagctg ggtagtgact actcgctatt caagaagaac
      421 attcgtccca tgtgggagga tgcagccaac aaacagggcg gtcgttgggt aattaccctt
      481 aacaaaagct ccaagaccga tctggataac ctatggctcg atgtgctgct ctgcctgatt
      541 ggtgaggcct tcgatcactc cgatcagatc tgcggcgctg ttataaacat tcgcggcaag
      601 agcaacaaga tatccatctg gactgccgac ggaaacaacg aggaagctgc ccttgagatt
      661 ggtcacaagc tgcgcgatgc cttgcgtctg ggacgcaaca actcgctgca gtatcagttg
      721 cacaaggaca cgatggtcaa gcagggctcc aacgtgaaat cgatctacac tttgtagggc
      781 ggccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa ccctctcctc
      841 ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta aacccgctga
      901 tcagcctcga ctgtgccttc taaggcctga gctcgctgat cagcctcgat cgaggatcca
      961 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     1021 tgctttattt gt
//
