LOCUS       sequence-153054-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged DmeIF4E in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Zekri L, Kuzuoglu-Ozturk D, Izaurralde E
  TITLE     GW182 proteins cause PABP dissociation from silenced miRNA targets 
            in the absence of deadenylation.
  JOURNAL   EMBO J. 2013 Apr 3;32(7):1052-65. doi: 10.1038/emboj.2013.44. Epub 
            2013 Mar 5.
  PUBMED    23463101
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/emboj.2013.44"; journalName: "EMBO J"; date: "2013-04-3- 
            3"; volume: "32"; issue: "7"; pages: "1052-65"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmeIF4E
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..45
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(68..85)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(95..136)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 tcgaggctga tcagcgagct caggccttag aaggcacagt cgaggctgat cagcgggttt
       61 aaactcaatg gtgatggtga tgatgaccgg tacgcgtaga atcgagaccg aggagagggt
      121 tagggatagg cttaccttcg aaccgcgggc cctctagact cgagcggccg ccctacaaag
      181 tgtagatcga tttcacgttg gagccctgct tgaccatcgt gtccttgtgc aactgatact
      241 gcagcgagtt gttgcgtccc agacgcaagg catcgcgcag cttgtgacca atctcaaggg
      301 cagcttcctc gttgtttccg tcggcagtcc agatggatat cttgttgctc ttgccgcgaa
      361 tgtttataac agcgccgcag atctgatcgg agtgatcgaa ggcctcacca atcaggcaga
      421 gcagcacatc gagccatagg ttatccagat cggtcttgga gcttttgtta agggtaatta
      481 cccaacgacc gccctgtttg ttggctgcat cctcccacat gggacgaatg ttcttcttga
      541 atagcgagta gtcactaccc agcttgatct ctgatggggg cttgatgtgg ttgtataggc
      601 tccagaagtc ctcgacggta tcgaagctgg tgatctcgtt ttgcatgtcc tcccaggact
      661 tggaccgatc gttttcaagg taccacagcg tccagacatt catgagcggg tgtttgtata
      721 aatgctcggt gcgcacagca tcgtcgccgg caggagcagt agtggttgca gtgttgcctg
      781 ctggttcacc agtctcctgt gggtcctcct tgggcttgac atccttagcc tcggcgggcg
      841 ctgcagccga agttggtggt tccggacgac cctgctcggt gctgggggcg ctggtcttct
      901 ccgtctccaa cactaccata gaattcaagc ttggcttgta cagctcgtcc atgccgagag
      961 tgatcccggc ggcggtcacg aactccagca gg
//
