LOCUS       sequence-153058-         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Hogg M, Seki M, Wood RD, Doublie S, Wallace SS
  TITLE     Lesion bypass activity of DNA polymerase theta (POLQ) is an 
            intrinsic property of the pol domain and depends on unique sequence 
            inserts.
  JOURNAL   J Mol Biol. 2011 Jan 21;405(3):642-52. doi: 
            10.1016/j.jmb.2010.10.041. Epub 2010 Nov 2.
  PUBMED    21050863
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2010.10.041"; journalName: "J Mol Biol"; date: 
            "2011-01-21- 21"; volume: "405"; issue: "3"; pages: "642-52"
COMMENT     Sequence Label: Sumo3 PolQM1
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..344
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 3"
                     /label=SUMO3
                     /translation="EEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYC
                     ERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatgaa aggtcatcac catcatcatc
       61 acgggtccct gcaggaggag aagcccaagg agggtgtgaa gacagagaat gaccacatca
      121 acctgaaggt ggccgggcag gacggctccg tggtgcagtt caagatcaag aggcacacgc
      181 cgctgagcaa gctgatgaag gcctactgcg agaggcaggg cttgtcaatg aggcagatca
      241 gattcaggtt cgacgggcag ccaatcaatg aaactgacac tccagcacag ctggagatgg
      301 aggacgagga caccatcgac gtgttccagc agcagacggg aggtgggttc aaagacaaca
      361 gccctattag tgacacaagc ttttcacttc agttatcaca ggatggatta cagttaactc
      421 cagcctcaag cagttcagaa agtttgtcca taattgatgt agcaagtgac caaaatcttt
      481 tccaaacatt cattaaggag tggcggtgca aaaagcgatt ttccatctca ctggcttgtg
      541 aaaagattag aagtttgaca tcttctaaaa ctgctactat tggcagtagg tttaagcaag
      601 ctagctcacc tcaggaaatt cctattagag atgatggatt tcccattaaa ggttgtgatg
      661 acaccttggt ggttggactg gcagtatgct ggggtggaag ggatgcctat tatttttcac
      721 tgcagaagga acaaaagcat tctgaaatta gtgccagttt ggttccacct tctttagatc
      781 caagcctgac tttgaaagac aggatgtggt accttcaatc ttgcttgcga aaggaatctg
      841 ataaagaatg ttctgttgtc atctatgact tcatccagag ctataaaatt cttcttcttt
      901 cttgtggcat ctccttggag caaagttatg aagatcctaa ggtggcatgc tggttactag
      961 atccagattc tcaggagccg actcttcata gc
//
