LOCUS       sequence-153061-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Hogg M, Seki M, Wood RD, Doublie S, Wallace SS
  TITLE     Lesion bypass activity of DNA polymerase theta (POLQ) is an 
            intrinsic property of the pol domain and depends on unique sequence 
            inserts.
  JOURNAL   J Mol Biol. 2011 Jan 21;405(3):642-52. doi: 
            10.1016/j.jmb.2010.10.041. Epub 2010 Nov 2.
  PUBMED    21050863
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2010.10.041"; journalName: "J Mol Biol"; date: 
            "2011-01-21- 21"; volume: "405"; issue: "3"; pages: "642-52"
COMMENT     Sequence Label: Sumo3 PolQM1 deltains1
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             71..340
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 3"
                     /label=SUMO3
                     /translation="EEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYC
                     ERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
ORIGIN
        1 gaatattttg tttactttaa gaaggagata taccatgggt catcaccatc atcatcacgg
       61 gtccctgcag gaggagaagc ccaaggaggg tgtgaagaca gagaatgacc acatcaacct
      121 gaaggtggcc gggcaggacg gctccgtggt gcagttcaag atcaagaggc acacgccgct
      181 gagcaagctg atgaaggcct actgcgagag gcagggcttg tcaatgaggc agatcagatt
      241 caggttcgac gggcagccaa tcaatgaaac tgacactcca gcacagctgg agatggagga
      301 cgaggacacc atcgacgtgt tccagcagca gacgggaggt gggttcaaag acaacagccc
      361 tattagtgac acaagctttt cacttcagtt atcacaggat ggattacagt taactccagc
      421 ctcaagcagt tcagaaagtt tgtccataat tgatgtagca agtgaccaaa atcttttcca
      481 aacattcatt aaggagtggc ggtgcaaaaa gcgattttcc atctcactgg cttgtgaaaa
      541 gattagaagt ttgacatctt ctaaaactgc tactattggc agtaggttta agcaagctag
      601 ctcacctcag gaaattccta ttagagatga tggatttccc attaaaggtt gtgatgacac
      661 cttggtggtt ggactggcag tatgctgggg tggaagggat gcctattatt tttcactgca
      721 gaaggaacaa aagcattctg aaattagtgc cagtttggtt ccaccttctt tagatccaag
      781 cctgactttg aaagacagga tgtggtacct tcaatcttgc ttgcgaaagg aatctgataa
      841 agaatgttct gttgtcatct atgacttcat ccagagctat aaaattcttc ttctttcttg
      901 tggcatctcc ttggagcaaa gttatgaaga tcctaaggtg gcatgctggt tactagatcc
      961 agattctcag gagccgactc ttcatagcat agtt
//
