LOCUS       sequence-153064-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged DmNot3 in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Haas G, Braun JE, Igreja C, Tritschler F, Nishihara T, Izaurralde E
  TITLE     HPat provides a link between deadenylation and decapping in metazoa.
  JOURNAL   J Cell Biol. 2010 Apr 19. 189(2):289-302.
  PUBMED    20404111
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2010 Apr 19. 189(2):289-302."
COMMENT     Sequence Label: pAc5.1B-EGFP-DmNot3
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..55
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(78..95)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(105..146)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ggatcctcga tcgaggctga tcagcgagct caggccttag aaggcacagt cgaggctgat
       61 cagcgggttt aaactcaatg gtgatggtga tgatgaccgg tacgcgtaga atcgagaccg
      121 aggagagggt tagggatagg cttaccttcg aaccgcgggc cctctagact cgagcggccg
      181 cctcaattca gctccttgtc ctctaagtac ttgtattcaa aggtgaatcc ctccttcttt
      241 cgctgactcc acttttcata atcaaaatag atgtacgtac cctgttcgta atcatcgttg
      301 ataatcttcg gctcctcgtg cctttgaaac cacatcatat actttgtgtg gaaacgccag
      361 ctctgctttt ttaaggcctt ggccgctaga tactgggcct tggagccctc catatagtag
      421 aacacaaaaa aaagggtctc cgttgagagt cgttgataaa actcaactgt atcatatatt
      481 ggcatctgtg cctggggata gtgcgatggc gtgagcactg gtgcgcgatg aaagtacgtt
      541 tgtagctttt ccgtgtccat aggctgtggc aggtgatagt aggcagcttc catcatttgg
      601 aactgcattt ggtgctcctt ttgaagtggt gttggcccaa gtggtgtcac accaagcagt
      661 gtgggaatgt gcgcttccgt cgtggccatc tgttggggtt ggcacgcatg cgttttggca
      721 ttgccgctga taggctgacc agtagcgttt gctacattca tgagaccact accagttgaa
      781 gttccgttgt tagaacctgt ggtcgcagca agggagttat tctgaagctg ttgctgctgc
      841 tgcgatgtca attgctgttg attcgtgttg gtttcagagt tgaaatgttg gagaagtgat
      901 ttctgcgact gcggctgtct cagatcgatg ctgcttgttt gctgctgatt cagtgaatta
      961 ggatcgatcg cagatcga
//
