LOCUS       sequence-153065-         973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Hogg M, Seki M, Wood RD, Doublie S, Wallace SS
  TITLE     Lesion bypass activity of DNA polymerase theta (POLQ) is an 
            intrinsic property of the pol domain and depends on unique sequence 
            inserts.
  JOURNAL   J Mol Biol. 2011 Jan 21;405(3):642-52. doi: 
            10.1016/j.jmb.2010.10.041. Epub 2010 Nov 2.
  PUBMED    21050863
REFERENCE   2  (bases 1 to 973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jmb.2010.10.041"; journalName: "J Mol Biol"; date: 
            "2011-01-21- 21"; volume: "405"; issue: "3"; pages: "642-52"
COMMENT     Sequence Label: Sumo3 PolQM1 deltains2
FEATURES             Location/Qualifiers
     source          1..973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             42..59
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             72..341
                     /codon_start=1
                     /product="human small ubiquitin-related modifier 3"
                     /label=SUMO3
                     /translation="EEKPKEGVKTENDHINLKVAGQDGSVVQFKIKRHTPLSKLMKAYC
                     ERQGLSMRQIRFRFDGQPINETDTPAQLEMEDEDTIDVFQQQTGG"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg tcatcaccat catcatcacg
       61 ggtccctgca ggaggagaag cccaaggagg gtgtgaagac agagaatgac cacatcaacc
      121 tgaaggtggc cgggcaggac ggctccgtgg tgcagttcaa gatcaagagg cacacgccgc
      181 tgagcaagct gatgaaggcc tactgcgaga ggcagggctt gtcaatgagg cagatcagat
      241 tcaggttcga cgggcagcca atcaatgaaa ctgacactcc agcacagctg gagatggagg
      301 acgaggacac catcgacgtg ttccagcagc agacgggagg tgggttcaaa gacaacagcc
      361 ctattagtga cacaagcttt tcacttcagt tatcacagga tggattacag ttaactccag
      421 cctcaagcag ttcagaaagt ttgtccataa ttgatgtagc aagtgaccaa aatcttttcc
      481 aaacattcat taaggagtgg cggtgcaaaa agcgattttc catctcactg gcttgtgaaa
      541 agattagaag tttgacatct tctaaaactg ctactattgg cagtaggttt aagcaagcta
      601 gctcacctca ggaaattcct attagagatg atggatttcc cattaaaggt tgtgatgaca
      661 ccttggtggt tggactggca gtatgctggg gtggaaggga tgcctattat ttttcactgc
      721 agaaggaaca aaagcattct gaaattagtg ccagtttggt tccaccttct ttagatccaa
      781 gcctgacttt gaaagacagg atgtggtacc ttcaatcttg cttgcgaaag gaatctgata
      841 aagaatgttc tgttgtcatc tatgacttca tccagagcta taaaattctt cttctttctt
      901 gtggcatctc cttggagcaa agttatgaag atcctaaggt ggcatgctgg ttactagatc
      961 cagattctca gga
//
