LOCUS       sequence-153068-        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged DmCCr4 in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Haas G, Braun JE, Igreja C, Tritschler F, Nishihara T, Izaurralde E
  TITLE     HPat provides a link between deadenylation and decapping in metazoa.
  JOURNAL   J Cell Biol. 2010 Apr 19. 189(2):289-302.
  PUBMED    20404111
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2010 Apr 19. 189(2):289-302."
COMMENT     Sequence Label: pAc5.1B-EGFP-DmCCr4
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..54
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(77..94)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(104..145)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gatcctcgat cgaggctgat cagcgagctc aggccttaga aggcacagtc gaggctgatc
       61 agcgggttta aactcaatgg tgatggtgat gatgaccggt acgcgtagaa tcgagaccga
      121 ggagagggtt agggataggc ttaccttcga accgcgggcc ctctagatta ccggcgattg
      181 atcagcccgt taggaggcgc ctgttggcta gccgtatgca tcagctctag ctcgaccagc
      241 agtgggaagt gatcagaggg tatatgcgga tgtgggcatc caacaacctt attctcgcgc
      301 agccaatcat tggagacagg acccagcagg cccagcggca ccatgcccgt cttcgtgtag
      361 aaaatgtagt cgatgatgcc cttaaaatcg aacgtatagt tggtgtgcgg catgatgtcc
      421 tcgttgtagg cggaggctag cttgaacgag tgcgtaaact cgttggtgtc gttcgagagc
      481 agccgctgca ggcaggactt gtagcccatg tccttgaagt ccaaatgatc catggaaacg
      541 cggcccttgc cgagaaactc cacaacgcct gaatcgggta gcgagttgaa gtcaccgcac
      601 agcagcagct ggacagcatt ggagtcgttc ttgtgaccag gtcggaaact gtggctcgcc
      661 tcgtcgatga tcgtcttcaa ctcattgcta agcatcatcg tctggatgag cttgacgtcg
      721 cagaactcag ggtcccagtg tatgtgcgcc gtgcagacga gcagcggctg cgagatctgc
      781 gtcacctcgg acatcggctc ccatgcgttc tccttcacct tgagcagtgc ggccagaccg
      841 atgttatcct taggcattac gcggttcagc atgttgtcgg agccctcggc attggccatt
      901 gccagctgat tgaactcgat cagtgattcc ttgatcagcg taaacttgga cgccctgaag
      961 aatatcgcac agccatcgac gtacttcctc tccagctcgg acatagtctt agcacgcgac
     1021 ttcggtgaga agattcc
//
