LOCUS       sequence-153073-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged DmNot11 in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Bawankar P, Loh B, Wohlbold L, Schmidt S, Izaurralde E
  TITLE     NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT 
            complex that docks onto the NOT1 N-terminal domain.
  JOURNAL   RNA Biol. 2013 Feb;10(2):228-44. doi: 10.4161/rna.23018. Epub 2013 
            Jan 9.
  PUBMED    23303381
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/rna.23018";
            journalName: "RNA Biol"; date: "2013-02"; volume: "10"; issue: "2"; 
            pages: "228-44"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmNot11
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..59
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(82..99)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(109..150)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(914..935)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtctggatcc tcgatcgagg ctgatcagcg agctcaggcc ttagaaggca cagtcgaggc
       61 tgatcagcgg gtttaaactc aatggtgatg gtgatgatga ccggtacgcg tagaatcgag
      121 accgaggaga gggttaggga taggcttacc ttcgaaccgc gggccctcta gactacttag
      181 ctagggagtt ggacgacaga gcgctttccc cagtttccaa gtgtttgata agccggtaca
      241 gggtagcagc ctccttgatt ttgctgaaac ccacgcagaa ggcttcgatt tcaatgaata
      301 gttccctaac gttcacaatc ttgttcctaa tcaaggactg cagaaatacg caaacgagtc
      361 gcaccaggcg agactgcatg tacttgtcct tgactgtttc gcaagtcgag atgcaattgc
      421 tgatgtatag gtgaataaac tcagtgggca aaggtccgga ggtggttagt cggttgacca
      481 cctccatgga gtgaagtgtt atgtccatgt tgaccaacac gttgaagtat tcagttatgt
      541 cggaggtcgt catcagtctt agcaagatct caatggagat gagaggattg ctttccacga
      601 gcagaggtag cttgtccggg gtcagtccat attggcgcgc aatgtccgtg ttctgcttca
      661 gcaggtcgtc gaagaggatg tgctggttct ggaggctgag tggctggaga aaagcctgct
      721 cgatgaggtg ctttatgttg ggggtaatgt agttggactt gtcgtagatg atctccacct
      781 tgtcgctgtt cgactggttt atccagagca gctcgtcctc gaagtccatt aggggcgggg
      841 cgatggacag aaacatctta attaagcttg gcttgtacag ctcgtccatg ccgagagtga
      901 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
      961 tgctcagggc ggactgggtg ctcaggtagt ggttgtc
//
