LOCUS       sequence-153077-        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses proteins in the cytoplasm as fusions to a high-binding 
            mutant (A313V) maltose-binding protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Walker IH, Hsieh PC, Riggs PD
  TITLE     Mutations in maltose-binding protein that alter affinity and 
            solubility properties.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(1):187-97. doi: 
            10.1007/s00253-010-2696-y. Epub 2010 Jun 10.
  PUBMED    20535468
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2696-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "1"; pages: 
            "187-97"
COMMENT     Sequence Label: pMAL-c4X
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     116..133
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     297..319
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     311..328
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     312..328
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(376..387)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(439..462)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 cacactacca tcggcgctac ggcgtttcac ttctgagttc ggcatggggt caggtgggac
       61 caccgcgcta ctgccgccag gcaaattctg ttttatcaga ccgcttctgc gttctgattt
      121 aatctgtatc aggctgaaaa tcttatctca tccgccaaaa cagccaagct gccattcgcc
      181 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
      241 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
      301 gtcacgacgt tgtaaaacga cggccagtgc caagcttgcc tgcaggtcga ctctagagga
      361 tccgaattct gaaatccttc cctcgatccc gaggttgttg ttattgttat tgttgttgtt
      421 gttcgagctc gaattagtct gcgcgtcttt cagggcttca tcgacagtct gacgaccgct
      481 ggcggcgttg atcaccgcag tacgcacggc ataccagaaa gcggacatct gcgggatgtt
      541 cggcatgatt tcacctttct gggcgttttc catagtggcg gcaatccgcg gatctttcac
      601 caactcttcc tcgtaagact tcagcgctac ggcacccagc ggtttgtctt tattaaccgc
      661 ttccagacct tcatcagtca gcagatagtt ttcgaggaac tcttttgcca gctctttgtt
      721 cggactggcg gcgttaatac ctgcgctcag cacgccaacg aacggtttgg atggttgacc
      781 cttgaaggtc ggcagtaccg ttacaccata attcactttg ctggtgtcga tgttggacca
      841 tgcccacggg ccgttgatgg tcatcgctgt ttcgccttta ttaaaggcag cttctgcgat
      901 ggagtaatcg gtgtctgcat tcatgtgttt gtttttaatc aggtcaacca ggaaggtcag
      961 acccgctttc gcgccagcgt tatccacgcc cacgtcttta atgtcgtact tgccgttttc
     1021 atacttgaac gcat
//
