LOCUS       sequence-153086-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged DmNot10 in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Bawankar P, Loh B, Wohlbold L, Schmidt S, Izaurralde E
  TITLE     NOT10 and C2orf29/NOT11 form a conserved module of the CCR4-NOT 
            complex that docks onto the NOT1 N-terminal domain.
  JOURNAL   RNA Biol. 2013 Feb;10(2):228-44. doi: 10.4161/rna.23018. Epub 2013 
            Jan 9.
  PUBMED    23303381
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.4161/rna.23018";
            journalName: "RNA Biol"; date: "2013-02"; volume: "10"; issue: "2"; 
            pages: "228-44"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmNot10
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..55
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(78..95)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(105..146)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ggatcctcga tcgaggctga tcagcgagct caggccttag aaggcacagt cgaggctgat
       61 cagcgggttt aaactcaatg gtgatggtga tgatgaccgg tacgcgtaga atcgagaccg
      121 aggagagggt tagggatagg cttaccttcg aaccgcgggc cctctagact aactcggcgt
      181 cccgccagga acgggaccca ttttgaggtc catgaagcgt cgcaaagcaa gcgccttgtt
      241 ggcaaccagc gaatgagtta aattggccag gagtgcttta gcttgaggca ggtcgttctg
      301 tagagccatg gcaacggcca gattataccg cacaacgttc tgagcggcgt ccaccgactt
      361 cagttgccag tcacgcgtct caaaatcgaa ggcgtttagt gtgcccacaa aagtagggtc
      421 caaatggtcg cgtgcctcgc tggccttgtc cataagcatc aacgcctcgc cagcgtacat
      481 gtgacctagc aatttgtgtg catcggacaa gcgttcacag gccagcagtt tttccgacat
      541 ctcgagcgcg gtcacatgat cgcctaggcg cagcgagacg aagctgtgag cagcatatat
      601 ggcagccatc atgttttcca aagactctag gctgactggc ttcgagggat tgcagaagtt
      661 attgtcctgg ggatgacgcc aactctcctg agttgggtcc ttgggttcgg gtgcttccac
      721 atcctccgag gagaccgccg ccatgtgaaa cctcgtcttg tagtgctgcg taagcgttaa
      781 cgcgctacgt agacacaaga cagcgaattc cagcgtgggc tctggaactc ccgccgattg
      841 aggagcgtat ggctttgttg atggcgtttc tgactgcgac tggcgctcct cctccactag
      901 tttcgcctca tgctccatga tgcaggcctc agccatgcgc agccaaagac gtggattgct
      961 gtggaattgc ttaacgggaa ccagaaagca ctgaaac
//
