LOCUS       sequence-153087-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses proteins in the periplasm as fusions to a high-binding 
            mutant (A313V) maltose-binding protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Walker IH, Hsieh PC, Riggs PD
  TITLE     Mutations in maltose-binding protein that alter affinity and 
            solubility properties.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(1):187-97. doi: 
            10.1007/s00253-010-2696-y. Epub 2010 Jun 10.
  PUBMED    20535468
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2696-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "1"; pages: 
            "187-97"
COMMENT     Sequence Label: pMAL-p4X
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(142..147,148..163,164..170)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    178..194
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     183..205
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcat aattcgtgtc
       61 gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata acggttctgg
      121 caaatattct gaaatgagct gttgacaatt aatcatcggc tcgtataatg tgtggaattg
      181 tgagcggata acaatttcac acaggaaaca gccagtccgt ttaggtgttt tcacgagcac
      241 ttcaccaaca aggaccatag catatgaaaa taaaaacagg tgcacgcatc ctcgcattat
      301 ccgcattaac gacgatgatg ttttccgcct cggctctcgc caaaatcgaa gaaggtaaac
      361 tggtaatctg gattaacggc gataaaggct ataacggtct cgctgaagtc ggtaagaaat
      421 tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc ggataaactg gaagagaaat
      481 tcccacaggt tgcggcaact ggcgatggcc ctgacattat cttctgggca cacgaccgct
      541 ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg
      601 acaagctgta tccgtttacc tgggatgccg tacgttacaa cggcaagctg attgcttacc
      661 cgatcgctgt tgaagcgtta tcgctgattt ataacaaaga tctgctgccg aacccgccaa
      721 aaacctggga agagatcccg gcgctggata aagaactgaa agcgaaaggt aagagcgcgc
      781 tgatgttcaa cctgcaagaa ccgtacttca cctggccgct gattgctgct gacgggggtt
      841 atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga cgtgggcgtg gataacgctg
      901 gcgcgaaagc gggtctgacc ttcctggttg acctgattaa aaacaaacac atgaatgcag
      961 acaccgatta ctccatcgca gaagctgcct ttaataaagg cgaaacagcg atgaccatca
     1021 acggcccgtg ggcatggtcc aacatc
//
