LOCUS       sequence-153109-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses proteins in the periplasm as fusions to a high-binding 
            mutant (A313V) maltose-binding protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Walker IH, Hsieh PC, Riggs PD
  TITLE     Mutations in maltose-binding protein that alter affinity and 
            solubility properties.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(1):187-97. doi: 
            10.1007/s00253-010-2696-y. Epub 2010 Jun 10.
  PUBMED    20535468
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2696-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "1"; pages: 
            "187-97"
COMMENT     Sequence Label: pMAL-p4X
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     117..134
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     298..320
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     312..329
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     313..329
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(377..388)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(440..463)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga
       61 ccaccgcgct actgccgcca ggcaaattct gttttatcag accgcttctg cgttctgatt
      121 taatctgtat caggctgaaa atcttatctc atccgccaaa acagccaagc tgccattcgc
      181 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
      241 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
      301 agtcacgacg ttgtaaaacg acggccagtg ccaagcttgc ctgcaggtcg actctagagg
      361 atccgaattc tgaaatcctt ccctcgatcc cgaggttgtt gttattgtta ttgttgttgt
      421 tgttcgagct cgaattagtc tgcgcgtctt tcagggcttc atcgacagtc tgacgaccgc
      481 tggcggcgtt gatcaccgca gtacgcacgg cataccagaa agcggacatc tgcgggatgt
      541 tcggcatgat ttcacctttc tgggcgtttt ccatagtggc ggcaatccgc ggatctttca
      601 ccaactcttc ctcgtaagac ttcagcgcta cggcacccag cggtttgtct ttattaaccg
      661 cttccagacc ttcatcagtc agcagatagt tttcgaggaa ctcttttgcc agctctttgt
      721 tcggactggc ggcgttaata cctgcgctca gcacgccaac gaacggtttg gatggttgac
      781 ccttgaaggt cggcagtacc gttacaccat aattcacttt gctggtgtcg atgttggacc
      841 atgcccacgg gccgttgatg gtcatcgctg tttcgccttt attaaaggca gcttctgcga
      901 tggagtaatc ggtgtctgca ttcatgtgtt tgtttttaat caggtcaacc aggaaggtca
      961 gacccgcttt cgcgccagcg ttatccacgc ccacgtcttt aatgtcgtac ttgccgtttt
     1021 catac
//
