LOCUS       sequence-153116-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses proteins in the cytoplasm as fusions to a high-binding 
            mutant (A313V) maltose-binding protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Walker IH, Hsieh PC, Riggs PD
  TITLE     Mutations in maltose-binding protein that alter affinity and 
            solubility properties.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(1):187-97. doi: 
            10.1007/s00253-010-2696-y. Epub 2010 Jun 10.
  PUBMED    20535468
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2696-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "1"; pages: 
            "187-97"
COMMENT     Sequence Label: pMAL-c4E
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(156..161,162..177,178..184)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    192..208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     197..219
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact
       61 gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat
      121 cataacggtt ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat
      181 aatgtgtgga attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt
      241 gttttcacga gcacttcacc aacaaggacc atagcatatg aaaatcgaag aaggtaaact
      301 ggtaatctgg attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt
      361 cgagaaagat accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt
      421 cccacaggtt gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt
      481 tggtggctac gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga
      541 caagctgtat ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc
      601 gatcgctgtt gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa
      661 aacctgggaa gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct
      721 gatgttcaac ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta
      781 tgcgttcaag tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg
      841 cgcgaaagcg ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga
      901 caccgattac tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa
      961 cggcccgtgg gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact
     1021 gccgaccttc aaggg
//
