LOCUS       sequence-153117-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses proteins in the cytoplasm as fusions to a high-binding 
            mutant (A313V) maltose-binding protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Walker IH, Hsieh PC, Riggs PD
  TITLE     Mutations in maltose-binding protein that alter affinity and 
            solubility properties.
  JOURNAL   Appl Microbiol Biotechnol. 2010 Sep;88(1):187-97. doi: 
            10.1007/s00253-010-2696-y. Epub 2010 Jun 10.
  PUBMED    20535468
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00253-010-2696-y"; journalName: "Appl Microbiol 
            Biotechnol"; date: "2010-09"; volume: "88"; issue: "1"; pages: 
            "187-97"
COMMENT     Sequence Label: pMAL-c4E
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     117..134
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     298..320
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     312..329
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     313..329
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(377..391)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     primer_bind     complement(443..466)
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
ORIGIN
        1 ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg tcaggtggga
       61 ccaccgcgct actgccgcca ggcaaattct gttttatcag accgcttctg cgttctgatt
      121 taatctgtat caggctgaaa atcttatctc atccgccaaa acagccaagc tgccattcgc
      181 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
      241 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
      301 agtcacgacg ttgtaaaacg acggccagtg ccaagcttgc ctgcaggtcg actctagagg
      361 atccgaattc cggtaccttg tcatcgtcat ccccgaggtt gttgttattg ttattgttgt
      421 tgttgttcga gctcgaatta gtctgcgcgt ctttcagggc ttcatcgaca gtctgacgac
      481 cgctggcggc gttgatcacc gcagtacgca cggcatacca gaaagcggac atctgcggga
      541 tgttcggcat gatttcacct ttctgggcgt tttccatagt ggcggcaatc cgcggatctt
      601 tcaccaactc ttcctcgtaa gacttcagcg ctacggcacc cagcggtttg tctttattaa
      661 ccgcttccag accttcatca gtcagcagat agttttcgag gaactctttt gccagctctt
      721 tgttcggact ggcggcgtta atacctgcgc tcagcacgcc aacgaacggt ttggatggtt
      781 gacccttgaa ggtcggcagt accgttacac cataattcac tttgctggtg tcgatgttgg
      841 accatgccca cgggccgttg atggtcatcg ctgtttcgcc tttattaaag gcagcttctg
      901 cgatggagta atcggtgtct gcattcatgt gtttgttttt aatcaggtca accaggaagg
      961 tcagacccgc tttcgcgcca gcgttatcca cgcccacgtc tttaatgtcg tacttgccgt
     1021 tt
//
