LOCUS       sequence-153118-         779 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Suicide vector for gene replacement, modified MCS to maximise 
            utility of insert restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Quandt J, Hynes MF
  TITLE     Versatile suicide vectors which allow direct selection for gene 
            replacement in gram-negative bacteria.
  JOURNAL   Gene. 1993 May 15;127(1):15-21.
  PUBMED    8486283
REFERENCE   2  (bases 1 to 779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene."; 
            date: "1993-05-15"; volume: "127(1)"; pages: "15-21"
COMMENT     Sequence Label: pJQ200uc1
FEATURES             Location/Qualifiers
     source          1..779
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aagttgggca tacgggaaga agtgatgcac tttgatatcg acccaagtac cgccacctaa
       61 caattcgttc aagccgagat cggcttcccg gccgcggagt tgttcggtaa attgtcacaa
      121 cgccgcggcc atcggcattt tcttttgcgt ttttatttgt taactgttaa ttgtccttgt
      181 tcaaggatgc tgtctttgac aacagatgtt ttcttgcctt tgatgttcag caggaagctt
      241 ggcgcaaacg ttgattgttt gtctgcgtag aatcctctgt ttgtcatata gcttgtaatc
      301 acgacattgt ttcctttcgc ttgaggtaca gcgaagtgtg agtaagtaaa ggttacatcg
      361 ttaggatcaa gatccatttt taacacaagg ccagttttgt tcagcggctt gtatgggcca
      421 gttaaagaat tagaaacata accaagcatg taaatatcgt tagacgtaat gccgtcaatc
      481 gtcatttttg atccgcggga gtcagtgaac aggtaccatt tgccgttcat tttaaagacg
      541 ttcgcgcgtt caatttcatc tgttactgtg ttagatgcaa tcagcggttt catcactttt
      601 ttcagtgtgt aatcatcgtt tagctcaatc ataccgagag cgccgtttgc taactcagcc
      661 gtgcgttttt tatcgctttg cagaagtttt tgactttctt gacggaagaa tgatgtgctt
      721 ttgccatagt atgctttgtt aaataaagat tcttcgcctt ggtagccatc ttcagttcc
//
