LOCUS       sequence-153120-         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Modified RP4 plasmid with Km resistance and sacB gene. Easily 
            curable helper plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Quandt J, Clark RG, Venter AP, Clark SR, Twelker S, Hynes MF
  TITLE     Modified RP4 and Tn5-Mob derivatives for facilitated manipulation of
            large plasmids in Gram-negative bacteria.
  JOURNAL   Plasmid. 2004 Jul;52(1):1-12.
  PUBMED    15212888
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid."; 
            date: "2004-07"; volume: "52(1)"; pages: "1-12"
COMMENT     Sequence Label: RP4-11
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(106..561)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(243..264)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     455..474
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      complement(598..645)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 atgagttttt ctaagaatta attcatgagc ggatacatat ttgaatgtat ttagaaaaat
       61 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta
      121 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
      181 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
      241 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
      301 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg
      361 ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
      421 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
      481 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
      541 atgcgccgct acagggcgcg tcccattcgc caatccggat atagttcctc ctttcagcaa
      601 aaaacccctc aagacccgtt tagaggcccc aaggggttat gctagttatt gctcag
//
