LOCUS       sequence-153122-       10152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Tricistronic expression of TRPV4, ferritin, and mCherry in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, 
            Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann 
            CD, Guler AD
  TITLE     Genetically targeted magnetic control of the nervous system.
  JOURNAL   Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265.
  PUBMED    26950006
REFERENCE   2  (bases 1 to 10152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265."
COMMENT     Sequence Label: pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry
FEATURES             Location/Qualifiers
     source          1..10152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3520..3543
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3559..3615
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3649..3672
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4243..4266
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4861..4884
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4924..4980
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4987..5694
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(5129..5147)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5397..5416
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(5704..5722)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(5705..5722)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5742..5759)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5748..5972
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6018..6446
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6105..6124)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6315..6336
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6455..6475)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6460..6790
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6641..6776
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6703..6722
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6857..7651
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6911..6930)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7521..7540
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7825..7946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7862..7881)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7916..7935
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7995..8011)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7995..8011)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8008..8030)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8019..8035
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8043..8049,8050..8067,8068..8073))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8088..8109
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8226..8243)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8397..8985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8477..8496)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9156..9947,9948..10016))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9779..9798
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10017..10121)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gccaccatgg cagatcctgg tgatggcccc cgtgcagcgc ctggggatgt ggctgagccc
      961 cctggagacg agagtggcac ttctggtggg gaggccttcc ccctctcttc cctggccaac
     1021 ctgtttgagg gagaggaagg ctcctcttct ctttcaccag tggatgctag ccgccctgct
     1081 ggccccgggg atggacgtcc aaacctgcgt atgaagttcc agggcgcttt ccgcaagggg
     1141 gttcccaacc ccattgacct gctggagtcc accctgtatg agtcctcagt agtgcctggg
     1201 cccaagaaag cgcccatgga ttcgttgttc gactatggca cttaccggca ccaccccagt
     1261 gacaacaaga gatggaggag gaaggtcgta gagaagcagc cacagagccc caaagctccc
     1321 gccccccagc caccccccat cctcaaagtc ttcaaccggc ccatcctctt tgacatcgtg
     1381 tcccggggct ccactgccga cctggacgga ctgctctcct acttgctgac ccacaagaag
     1441 cgcctgactg atgaggagtt ccgggaacca tccacaggga agacctgcct gcccaaggca
     1501 cttctgaact taagcaatgg ccgaaacgac accatcccag tgttgctgga cattgcggaa
     1561 cgcacgggca acatgcggga gttcatcaac tcgcccttca gagacatcta ctaccgaggg
     1621 cagacggcac tgcacatcgc cattgaacgg cgctgcaagc attacgtgga gctcctggtg
     1681 gcccagggag ccgatgtgca cgcgcaggcc cgagggcggt tcttccagcc caaggatgag
     1741 ggtggctact tctactttgg ggagctgccc ttgtccttgg cagcctgcac caaccagccg
     1801 cacatcgtca actacctgac agagaaccct cacaagaaag ccgatatgag gcgacaggac
     1861 tccagaggca acacggtgct ccacgcgctg gtggccatcg ctgacaacac ccgagagaac
     1921 accaagtttg tcaccaagat gtatgacctg ttgcttctca agtgctcccg cctcttccca
     1981 gacagcaacc tggagactgt gcttaacaat gacggtcttt cgcccctcat gatggctgcc
     2041 aagactggca agatcggggt ctttcagcac atcatccgac gggaggtgac agatgaggac
     2101 acacggcacc tgtctcgcaa gttcaaggac tgggcctacg ggcctgtgta ttcttctctc
     2161 tacgacctct cctccctgga tacgtgcggg gaggaagtgt ccgtgctgga gatcctggtt
     2221 tacaacagca agatcgagaa ccgccatgag atgctggctg tggagcccat taacgaactg
     2281 ctgagggaca agtggcgtaa gttcggggcc gtgtccttct acatcaacgt tgtctcctat
     2341 ctgtgtgcca tggtcatctt caccctcaca gcctactatc agccactgga gggcacgcca
     2401 ccctaccctt accgtaccac ggtggactac ctgaggctgg ctggtgaggt catcacgctc
     2461 ctcacaggag tcctgttctt ctttaccagt atcaaagact tgttcatgaa gaaatgccct
     2521 ggagtgaatt ctctcttcgt cgatggctcc ttccagttgc tctacttcat ctactcagtg
     2581 ctggtggttg tgtctgcggc gctctacctg gcagggatcg aggcctatct ggctgtgatg
     2641 gtctttgccc tggtcctggg ctggatgaat gccctttact tcacccgtgg gctgaagctg
     2701 acagggacct acagcatcat gattcagaag atcctcttca aagatctctt ccgctttctg
     2761 ctggtctacc tgctttttat gattggctat gcctcagctc tggtcaccct cctgaatccg
     2821 tgcaccaaca tgaaggtctg taacgaggac cagagcaact gcacggtgcc ctcatacccc
     2881 gcgtgccggg acagcgagac cttcagcgcc ttcctactgg acctcttcaa gctcaccatc
     2941 ggcatgggcg acctggagat gctgagcagc gctaagtacc ccgtggtctt cattctcctg
     3001 ctggttacct acatcatcct caccttcgtg ctcctgctga acatgctcat cgccctcatg
     3061 ggtgagaccg tgggccaggt gtccaaggag agcaagcaca tctggaagct gcagtgggcc
     3121 accaccatcc tggacatcga gcgctccttc cctgtgttcc tgaggaaggc cttccgctcc
     3181 ggagagatgg tgacagtggg caagagctcg gatggcactc cagaccgcag gtggtgcttc
     3241 agggtggacg aggtgaactg gtctcactgg aaccagaacc tgggcatcat taacgaggac
     3301 cccggcaaga gcgagatcta ccagtactat ggcttctccc ataccatggg gcgcctccgc
     3361 agggatcgct ggtcctcagt ggtgccccgc gtggtggagc tgaacaagaa ctcaggcaca
     3421 gatgaagtgg tggtccccct ggataaccta gggaacccca actgtgacgg ccaccagcaa
     3481 ggttatgctc ccaagtggag ggcggaggac gcaccactgg attacaagga cgatgacgat
     3541 aagggatccg gcagcggcgc caccaacttc agcctgctga agcaggccgg cgacgtggag
     3601 gagaaccccg gccccctcga gtccggagca cggggcgggg gcggttctga ttataaggac
     3661 gacgacgaca aaggaggagg cggctcctct cgggttatgg gctcccagat tcgtcagaat
     3721 tattccaccg acgtggaggc agccgtcaac agcctggtca atttgtacct gcaggcctcc
     3781 tacacctacc tctctctggg cttctatttc gaccgcgatg atgtggctct ggaaggcgtg
     3841 agccacttct tccgcgaatt ggccgaggag aagcgcgagg gctacgagcg tctcctgaag
     3901 atgcaaaacc agcgtggcgg ccgcgctctc ttccaggaca tcaagaagcc agctgaagat
     3961 gagtggggta aaaccccaga cgccatgaaa gctgccatgg ccctggagaa aaagctgaac
     4021 caggcccttt tggatcttca tgccctgggt tctgcccgca cggaccccca tctctgtgac
     4081 ttcctggaga ctcacttcct agatgaggaa gtgaagctta tcaagaagat gggtgaccac
     4141 ctgaccaacc tccacaggct gggtggcccg gaggctgggc tgggcgagta tctcttcgaa
     4201 aggctcactc tcaagcacga cgcccgcggc ggcggcggct ccgattataa agatgatgat
     4261 gataaaggcg gcggcggctc ctcccgcgtc atgacgaccg cgtccacctc gcaggtgcgc
     4321 cagaactacc accaggactc agaggccgcc atcaaccgcc agatcaacct ggagctctac
     4381 gcctcctacg tttacctgtc catgtcttac tactttgacc gcgatgatgt ggctttgaag
     4441 aactttgcca aatactttct tcaccaatct catgaggaga gggaacatgc tgagaaactg
     4501 atgaagctgc agaaccaacg aggtggccga atcttccttc aggatatcaa gaaaccagac
     4561 tgtgatgact gggagagcgg gctgaatgca atggagtgtg cattacattt ggaaaaaaat
     4621 gtgaatcagt cactactgga actgcacaaa ctggccactg acaaaaatga cccccatttg
     4681 tgtgacttca ttgagacaca ttacctgaat gagcaggtga aagccatcaa agaattgggt
     4741 gaccacgtga ccaacttgcg caagatggga gcgcccgaat ctggcttggc ggaatatctc
     4801 tttgacaagc acaccctggg agacagtgat aatgaaagcg cacgcggggg tggcgggagc
     4861 gactataaag acgacgatga caaagggggt ggaggctcct cccgagtggc tagcggcagc
     4921 ggcgccacga acttctctct gttaaagcaa gcaggagacg tggaagaaaa ccccggtccc
     4981 ggatccgtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     5041 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     5101 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     5161 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     5221 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     5281 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     5341 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     5401 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     5461 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     5521 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     5581 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     5641 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagggccc
     5701 tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag
     5761 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     5821 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     5881 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     5941 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     6001 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     6061 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     6121 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc ggggcatccc
     6181 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     6241 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     6301 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     6361 ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa aaaatgagct
     6421 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     6481 aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca attagtcagc
     6541 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     6601 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     6661 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     6721 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     6781 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     6841 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     6901 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     6961 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     7021 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     7081 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     7141 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     7201 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     7261 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     7321 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     7381 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     7441 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     7501 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     7561 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     7621 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     7681 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     7741 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     7801 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     7861 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     7921 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     7981 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     8041 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     8101 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     8161 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     8221 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     8281 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     8341 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     8401 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     8461 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8521 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8581 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8641 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8701 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8761 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8821 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8881 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8941 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     9001 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     9061 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     9121 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     9181 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     9241 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     9301 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     9361 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     9421 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     9481 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     9541 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     9601 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     9661 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     9721 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     9781 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     9841 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     9901 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     9961 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    10021 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    10081 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    10141 ccacctgacg tc
//
