LOCUS       sequence-153127-        6208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This construct was engineered for the purpose of cloning and 
            sequencing Focal Adhesion Kinase..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Schaller MD, Borgman CA, Cobb BS, Vines RR, Reynolds AB, Parsons JT
  TITLE     pp125FAK a structurally distinctive protein-tyrosine kinase 
            associated with focal adhesions.
  JOURNAL   Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):5192-6.
  PUBMED    1594631
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1992-06-1"; volume: "89(11)"; pages: 
            "5192-6"
COMMENT     Sequence Label: pBS-FAK
FEATURES             Location/Qualifiers
     source          1..6208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(94..113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     304..325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     588..610
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     602..619
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     603..619
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     677..693
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     677..693
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3978..3994)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3979..3995)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(4023..4041)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4062..4078)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4062..4078)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4075..4097)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4086..4102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4110..4116,4117..4134,4135..4140))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4155..4176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4293..4310)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4464..5052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4544..4563)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5223..6014,6015..6083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5846..5865
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6084..6188)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
       61 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct
      481 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
      541 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      601 ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctcta gaactagtgg atcccccggg ctgcaggaat tccggatgtg
      721 ctgggctaaa gaatatgaca gatacctagc atctagcaaa accatggcag cagcttacct
      781 tgatccaaac ttgaatcata caccaagttc aagtgcaaag acgcacctcg gtactgggat
      841 ggagcgttcc ccgggggcca tggagcgagt cctaaaggtt tttcactact ttgaaaacag
      901 cagcgagcca acgacgtggg ccagcattat ccggcatgga gatgctactg atgttcgagg
      961 cataatacag aagattgtgg actgtcacaa agtgaaaaat gtggcctgct atgggttgcg
     1021 actcagtcat ctgcagtctg aggaggttca ctggctgcac ctggacatgg gggtatccaa
     1081 tgtgagagag aaatttgaac tagcacatcc tccagaagaa tggaaatatg aactgagaat
     1141 tcggtacctg cccaaaggat ttctaaacca gttcactgag gacaaaccaa ctttaaattt
     1201 tttctatcag caggtgaaaa atgactatat gttagaaata gcagatcaag tggaccagga
     1261 aattgctttg aaactaggtt gccttgaaat caggagatcc tacggagaga tgagaggcaa
     1321 tgcattagag aagaaatcca actatgaagt gctagaaaaa gatgtcggtt taagacgatt
     1381 ttttccgaag agtttgctag attcagtgaa ggccaaaaca ctacgaaaat taatccaaca
     1441 gacatttcga caatttgcca acctcaacag agaagaaagt attttgaaat tctttgagat
     1501 cctctctcca gtgtacagat ttgacaagga atgcttcaag tgtgcccttg gttcaagctg
     1561 gattatttca gtggagctgg caattggccc agaggaagga atcagctacc ttacagacaa
     1621 gggtgcaaat ccaactcacc tggcagattt taatcaagta caaactattc agtattcaaa
     1681 cagtgaagac aaggacagaa aagggatgtt gcaactgaag atagctggtg cacctgagcc
     1741 tctgacagtg acagcaccat ccttaaccat tgcagagaat atggctgact tgatagacgg
     1801 atactgccga ctggtgaatg gagccacgca atcttttatt atcaggccac agaaagaagg
     1861 tgaaagagct ttaccatcaa taccaaagct ggccaacaat gagaagcaag gagtaaggtc
     1921 gcacacagtc tctgtatcag aaacagatga ctatgcagag ataatagatg aagaagatac
     1981 ttatacaatg ccatcaacca gagattatga aattcaaagg gagagaattg aactggggcg
     2041 ctgcattggt gaaggacagt ttggagatgt gcaccaagga atttacatga gtccggaaaa
     2101 tccagctatg gctgtagcaa tcaaaacatg taaaaactgc acctcagaca gcgttagaga
     2161 aaagttccta caagaagcct taacaatgcg tcagtttgat catcctcaca ttgtgaagct
     2221 cattggagtt attacagaaa acccagtgtg gataatcatg gagctctgta cacttggaga
     2281 gttgagatcg tttctgcaag taagaaaatt cagcttggac ctggcctccc tcatcctcta
     2341 cgcttaccag cttagcacag cacttgctta cctagagagc aaaagatttg tacatagaga
     2401 tattgctgct aggaacgtgc tggtatctgc cactgactgt gtgaaattgg gtgactttgg
     2461 cttatcccga tacatggaag acagtactta ctataaagct tccaaaggaa agttacctat
     2521 caaatggatg gctccagagt caatcaactt ccgacggttt acctcagcaa gcgatgtgtg
     2581 gatgtttggt gtgtgtatgt gggagatcct gatgcatggg gtaaagccct tccagggagt
     2641 gaaaaataat gatgttattg gtcggattga gaacggtgag cggctcccca tgcctccgaa
     2701 ctgccctccc accctctaca gccttatgac caagtgctgg gcatacgacc ctagtagacg
     2761 acccaggttt actgaactta aagcacaact cagtacaata ctggaggagg agaagctgca
     2821 gcaagaggaa cgaatgagaa tggaatccag gcgacaagtc acagtatcct gggactcagg
     2881 aggatcagat gaagctcctc ccaagcccag caggcctggt taccccagcc caaggtccag
     2941 tgaagggttt tatccgagtc ctcagcatat ggtacagcca aatcactacc aggtatctgg
     3001 ctactctggt tctcatggga taccagccat ggcaggcagc atttatcctg ggcaagcttc
     3061 tctcttggat caaacagatt cctggaacca tcgacctcag gaagtatcag catggcagcc
     3121 aaacatggag gattcgggca ctttggatgt acgaggaatg gggcaggttc tgcccacaca
     3181 tctcatggag gagaggttaa taagacaaca gcaagagatg gaagaagatc aacgctggct
     3241 tgagaaagag gaacgattcc tggtaatgaa acctgatgtg cggctctcca gaggcagcat
     3301 tgaacgggag gacggaggtc tccagggccc agctggtaac cagcacatat atcagcctgt
     3361 gggtaaacca gatcatgccg ctccaccaaa gaagccccct cgccctggag ccccccactt
     3421 gggcagcctc gcgagcctga acagccccgt ggacagctac aacgaaggcg tgaagatcaa
     3481 gccacaggaa atcagccctc ctcctacggc caacctggac cgctccaatg acaaagtcta
     3541 tgagaatgta accgggctgg tgaaagctgt catagagatg tccagtaaaa tacagccagc
     3601 tccgccagag gagtacgtgc ccatggtaaa ggaggttggc ttggcgctga gaaccttgct
     3661 agcaacagtg gatgagtcgc tgccagtgct tcctgcaagc acccacagag agattgagat
     3721 ggcccagaaa ctgctgaact ctgacctggc tgagctcatt aacaagatga agctggccca
     3781 gcagtacgtc atgaccagcc tgcagcagga gtacaagaag caaatgctga cggctgctca
     3841 cgctctggct gtggatgcca agaacttgct ggatgtcatc gatcaagcca gactgaaaat
     3901 gatcagccag tccaggcccc actaagcacc gagggaaggt ttcatctgtc tgttgtagaa
     3961 ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac cagcttttgt
     4021 tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct gtttcctgtg
     4081 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4141 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     4201 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     4261 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4321 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4381 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4441 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4501 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4561 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4621 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4681 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4741 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4801 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4861 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     4921 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4981 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5041 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5101 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5161 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5221 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5281 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5341 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5401 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5461 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5521 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5581 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5641 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5701 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5761 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5821 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5881 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5941 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6001 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6061 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6121 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6181 gttccgcgca catttccccg aaaagtgc
//
