LOCUS       sequence-153130-         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pX330 vector encoding SpCas9 and a chimeric guide RNA targeting Prnp
            coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS
  TITLE     Manipulating the Prion Protein Gene Sequence and Expression Levels 
            with CRISPR/Cas9.
  JOURNAL   PLoS One. 2016 Apr 29;11(4):e0154604. doi: 
            10.1371/journal.pone.0154604. eCollection 2016.
  PUBMED    27128441
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-04-29"; volume: "11(4):e0154604. doi"; pages: " 
            10.1371/journal.pone.0154604. eCollection 2016"
COMMENT     Sequence Label: pX330_HR_Prnp_3
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        96..344
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     96..116
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     267..286
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        366..441
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
       61 gttcctggcc ttttgctggc cttttgctca catgtgaggg cctatttccc atgattcctt
      121 catatttgca tatacgatac aaggctgtta gagagataat tggaattaat ttgactgtaa
      181 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg
      241 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt
      301 tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgcgaga ccgatgtgaa
      361 gatgagtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
      421 aaagtggcac cgagtcggtg cttttttgtt ttagagctag aaatagcaag ttaaaataag
      481 gctagtccgt ttttagcgcg tgcgccaatt ctgcagacaa atggctctag aggtacccgt
      541 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      601 gtcaatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      661 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      721 caatgacggt aaatggcccg cctggcattg tgcccagtac atgaccttat gggactttcc
      781 tacttggc
//
