LOCUS       sequence-153135-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pX330 vector encoding SpCas9 and a chimeric guide RNA targeting Prnp
            coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS
  TITLE     Manipulating the Prion Protein Gene Sequence and Expression Levels 
            with CRISPR/Cas9.
  JOURNAL   PLoS One. 2016 Apr 29;11(4):e0154604. doi: 
            10.1371/journal.pone.0154604. eCollection 2016.
  PUBMED    27128441
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-04-29"; volume: "11(4):e0154604. doi"; pages: " 
            10.1371/journal.pone.0154604. eCollection 2016"
COMMENT     Sequence Label: pX330_HR_Prnp_3
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..50)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tactttttct tttttgcctg gccggccttt ttcgtggccg ccggcctttt gtcgcctccc
       61 agctgagaca ggtcgatccg tgtctcgtac aggccggtga tgctctggtg gatcagggtg
      121 gcgtccagca cctctttggt gctggtgtac ctcttccggt cgatggtggt gtcaaagtac
      181 ttgaaggcgg caggggctcc cagattggtc agggtaaaca ggtggatgat attctcggcc
      241 tgctctctga tgggcttatc ccggtgcttg ttgtaggcgg acagcacttt gtccagatta
      301 gcgtcggcca ggatcactct cttggagaac tcgctgatct gctcgatgat ctcgtccagg
      361 tagtgcttgt gctgttccac aaacagctgt ttctgctcat tatcctcggg ggagcccttc
      421 agcttctcat agtggctggc caggtacagg aagttcacat atttggaggg cagggccagt
      481 tcgtttccct tctgcagttc gccggcagag gccagcattc tcttccggcc gttttccagc
      541 tcgaacaggg agtacttagg cagcttgatg atcaggtcct ttttcacttc tttgtagccc
      601 ttggcttcca gaaagtcgat gggattcttc tcgaagctgc ttctttccat gatggtgatc
      661 cccagcagct ctttcacact cttcagtttc ttggacttgc ccttttccac tttggccacc
      721 accagcacag aataggccac ggtggggctg tcgaagccgc cgtacttctt agggtcccag
      781 tccttctttc tggcgatcag cttatcgctg ttcctcttgg gcaggataga ctctttgctg
      841 aagccgcctg tctgcacctc ggtctttttc acgatattca cttggggcat gctcagcact
      901 ttccgcacgg tggcaaaatc ccggccctta tcccacacga tctccccggt ttcgccgttt
      961 gtctcgatca gaggccgctt ccggatctcg ccgttggcca gggtaatctc ggtc
//
