LOCUS       sequence-153139-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pX335 vector encoding SpCas9n and a chimeric guide RNA targeting 
            Prnp coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS
  TITLE     Manipulating the Prion Protein Gene Sequence and Expression Levels 
            with CRISPR/Cas9.
  JOURNAL   PLoS One. 2016 Apr 29;11(4):e0154604. doi: 
            10.1371/journal.pone.0154604. eCollection 2016.
  PUBMED    27128441
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-04-29"; volume: "11(4):e0154604. doi"; pages: " 
            10.1371/journal.pone.0154604. eCollection 2016"
COMMENT     Sequence Label: pX335_HR_Prnp_3
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        122..370
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     122..142
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     293..312
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        392..467
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
       61 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      121 tgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga
      181 gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag
      241 aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca
      301 tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg
      361 acgaaacacc gcgagaccga tgtgaagatg agttttagag ctagaaatag caagttaaaa
      421 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt tttgttttag
      481 agctagaaat agcaagttaa aataaggcta gtccgttttt agcgcgtgcg ccaattctgc
      541 agacaaatgg ctctagaggt acccgttaca taacttacgg taaatggccc gcctggctga
      601 ccgcccaacg acccccgccc attgacgtca atagtaacgc caatagggac tttccattga
      661 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      721 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattgtgcc
      781 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt a
//
