LOCUS       sequence-153142-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA 
            targeting Prnp coding sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS
  TITLE     Manipulating the Prion Protein Gene Sequence and Expression Levels 
            with CRISPR/Cas9.
  JOURNAL   PLoS One. 2016 Apr 29;11(4):e0154604. doi: 
            10.1371/journal.pone.0154604. eCollection 2016.
  PUBMED    27128441
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-04-29"; volume: "11(4):e0154604. doi"; pages: " 
            10.1371/journal.pone.0154604. eCollection 2016"
COMMENT     Sequence Label: pX459_HR_Prnp_3
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..77)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 cttcctctgc cctctccact gccgaattcc tttttctttt ttgcctggcc ggcctttttc
       61 gtggccgccg gccttttgtc gcctcccagc tgagacaggt cgatccgtgt ctcgtacagg
      121 ccggtgatgc tctggtggat cagggtggcg tccagcacct ctttggtgct ggtgtacctc
      181 ttccggtcga tggtggtgtc aaagtacttg aaggcggcag gggctcccag attggtcagg
      241 gtaaacaggt ggatgatatt ctcggcctgc tctctgatgg gcttatcccg gtgcttgttg
      301 taggcggaca gcactttgtc cagattagcg tcggccagga tcactctctt ggagaactcg
      361 ctgatctgct cgatgatctc gtccaggtag tgcttgtgct gttccacaaa cagctgtttc
      421 tgctcattat cctcggggga gcccttcagc ttctcatagt ggctggccag gtacaggaag
      481 ttcacatatt tggagggcag ggccagttcg tttcccttct gcagttcgcc ggcagaggcc
      541 agcattctct tccggccgtt ttccagctcg aacagggagt acttaggcag cttgatgatc
      601 aggtcctttt tcacttcttt gtagcccttg gcttccagaa agtcgatggg attcttctcg
      661 aagctgcttc tttccatgat ggtgatcccc agcagctctt tcacactctt cagtttcttg
      721 gacttgccct tttccacttt ggccaccacc agcacagaat aggccacggt ggggctgtcg
      781 aagccgccgt acttcttagg gtcccagtcc ttctttctgg cgatcagctt atcgctgttc
      841 ctcttgggca ggatagactc tttgctgaag ccgcctgtct gcacctcggt cttttt
//
