LOCUS       sequence-153146-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of constitutively active chick E2F4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Morillo SM, Abanto EP, Roman MJ, Frade JM
  TITLE     Nerve growth factor-induced cell cycle reentry in newborn neurons is
            triggered by p38MAPK-dependent E2F4 phosphorylation.
  JOURNAL   Mol Cell Biol. 2012 Jul;32(14):2722-37. doi: 10.1128/MCB.00239-12. 
            Epub 2012 May 14.
  PUBMED    22586272
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00239-12"; journalName: "Mol Cell Biol"; date: 
            "2012-07"; volume: "32"; issue: "14"; pages: "2722-37"
COMMENT     Sequence Label: pcDNA6-E2F4CA-Myc
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    113..337
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     326..343
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(366..383)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(393..434)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(495..524)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
       61 ggcgcgtggg gataccccct agagccccag ctggttcttt ccgcctcaga agccatagag
      121 cccaccgcat ccccagcatg cctgctattg tcttcccaat cctccccctt gctgtcctgc
      181 cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc aatttcctca
      241 ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg cacgggggag
      301 gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca gcgggtttaa
      361 actcaatggt gatggtgatg atgaccggta cgcgtagaat cgagaccgag gagagggtta
      421 gggataggct taccttcgaa gggccctcta gactcgagcg gccgccactg tgctggatat
      481 ctgcagaatt ctcacagatc ctcttcagag atgagtttct gctcgaggtt aaggacaggc
      541 acatcgaaga ggtcacagac gccttcgctc tcatccaggt tgtagatgta gtcgtgatct
      601 ccaggaggag gggagaggcg gagcaaggga gcaaacactt cagatgacat cagctcctcc
      661 agcagctcag agttcataca ttctctcgtt ggatcaaaca tctctgacag ctctttggga
      721 agctccagca ttcctgcagg atcaggtttg attggctcaa aggaggcaga ggggctgggt
      781 aggacggagc tgctgtccag cgaggcagag gactgcagca gctgtgtgtc cagcctggct
      841 gtatggccgc tgggtgcaga cacattgctg agggggtcaa ggtcactgct gctgctcttg
      901 gactccacca ctgagatgct gcattctgtg ctcgtgtctt tctgctcagg agggctgtga
      961 tcctgagtgc tggaaggttc tggc
//
