LOCUS       sequence-153148-         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes sgRNA2.0 targeting 158nt upstream of TSS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS
  TITLE     Manipulating the Prion Protein Gene Sequence and Expression Levels 
            with CRISPR/Cas9.
  JOURNAL   PLoS One. 2016 Apr 29;11(4):e0154604. doi: 
            10.1371/journal.pone.0154604. eCollection 2016.
  PUBMED    27128441
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-04-29"; volume: "11(4):e0154604. doi"; pages: " 
            10.1371/journal.pone.0154604. eCollection 2016"
COMMENT     Sequence Label: sgRNA(MS2)_Prnp_SAM1
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        125..373
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     125..145
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     296..315
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        412..430
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        482..500
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     rep_origin      612..992
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(699..718)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     909..930
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcagc
      121 tagcgagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag
      181 agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
      241 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
      301 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
      361 aggacgaaac accgatagtt gctgagcgtc gtcagtttta gagctaggcc aacatgagga
      421 tcacccatgt ctgcagggcc tagcaagtta aaataaggct agtccgttat caacttggcc
      481 aacatgagga tcacccatgt ctgcagggcc aagtggcacc gagtcggtgc tttttttgaa
      541 ttctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
      601 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
      661 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
      721 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      781 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
      841 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      901 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
      961 ttttgattta taagggattt tgccgatttc ggtctattg
//
