LOCUS       sequence-153152-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac backbone, CAG promoter driving murine Tfe3, Hygromycin 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Betschinger J, Nichols J, Dietmann S, Corrin PD, Paddison PJ, Smith 
            A
  TITLE     Exit from pluripotency is gated by intracellular redistribution of 
            the bHLH transcription factor Tfe3.
  JOURNAL   Cell. 2013 Apr 11;153(2):335-47. doi: 10.1016/j.cell.2013.03.012.
  PUBMED    23582324
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.03"; journalName: "Cell"; date: "2013-04-11- 
            11"; volume: "153"; issue: "2"; pages: "335-47"
COMMENT     Sequence Label: pPB-CAG-Tfe3-pgk-hph
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..95
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(110..115,116..133,134..140)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    148..164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     153..175
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     172..188
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     172..188
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    209..233
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aggcacagtc gaggctgatc cgctggcacg acaggtttcc cgactggaaa gcgggcagtg
       61 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      121 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
      181 gctatgacca tgattacgcc aagctatcac cactttgtac aagaaagctg ggtctcagga
      241 ctcctcttcc atgctgaagc tgctacggcg gctgctggcc ttggaaacag ccggggatac
      301 tgaagaaagc agggggtcag aagcagcccg cagtggggac agggcacccc ctgacagtcc
      361 tcccaccatc ccctcctcct ccatcagaat gtcctctagc cctaggtgga aggggtcccc
      421 caggtctccc aagtggtcgc tgggaaagtg taggtccaga agggcatccg aaggtggtgc
      481 tggaggctgt tgtggaggag cgttctgggc aggtcctccc gatacatgga acgttgtgct
      541 aggcctgccc tcctcctcaa tgtccagctg ttctggcttg aggctgtcag agaccgaact
      601 cgtggttagg gagagcagtc ctgggttggg agggactggc agaccatgga tctgggcctg
      661 cagttctagc tcctgaatcc ggagctgcag gcttcggttg gcttgttcca gggaccgctg
      721 ccggctctcc aggtctttgg agcgttgctg ttccttctgt aatttgcgga tgtaatccac
      781 agatgccttc aggatggtg
//
