LOCUS       sequence-153154-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes sgRNA2.0 targeting 138nt upstream of TSS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Kaczmarczyk L, Mende Y, Zevnik B, Jackson WS
  TITLE     Manipulating the Prion Protein Gene Sequence and Expression Levels 
            with CRISPR/Cas9.
  JOURNAL   PLoS One. 2016 Apr 29;11(4):e0154604. doi: 
            10.1371/journal.pone.0154604. eCollection 2016.
  PUBMED    27128441
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-04-29"; volume: "11(4):e0154604. doi"; pages: " 
            10.1371/journal.pone.0154604. eCollection 2016"
COMMENT     Sequence Label: sgRNA(MS2)_Prnp_SAM2
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        125..373
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     125..145
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     296..315
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        411..429
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        481..499
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     rep_origin      611..991
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(698..717)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     908..929
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
       61 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcagc
      121 tagcgagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag
      181 agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
      241 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
      301 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
      361 aggacgaaac accgggagtg ctgacactgg ggggttttag agctaggcca acatgaggat
      421 cacccatgtc tgcagggcct agcaagttaa aataaggcta gtccgttatc aacttggcca
      481 acatgaggat cacccatgtc tgcagggcca agtggcaccg agtcggtgct ttttttgaat
      541 tctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
      601 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      661 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
      721 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      781 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
      841 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
      901 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
      961 tttgatttat aagggatttt gccgatttcg gtctattggt t
//
