LOCUS       sequence-153157-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac for EOS-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, 
            Santos F, Clarke J, Mansfield W, Reik W, Bertone P, Smith A
  TITLE     Resetting Transcription Factor Control Circuitry toward Ground-State
            Pluripotency in Human.
  JOURNAL   Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
  PUBMED    25215486
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.08"; journalName: "Cell"; date: "2014-09-11- 
            11"; volume: "158"; issue: "6"; pages: "1254-69"
COMMENT     Sequence Label: pPB-EOS-GFP-IP
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     repeat_region   18..80
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    complement(251..284)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     308..324
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 aagctggagc tctttaaccc tagaaagata atcatattgt gacgtacgtt aaagataatc
       61 atgcgtaaaa ttgacgcatg tgttttatcg gtctgtatat cgaggtttat ttattaattt
      121 gaatagatat taagttttat tatatttaca cttacatact aataataaat tcaacaaaca
      181 atttatttat gtttatttat ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa
      241 gtaacaaaac ataacttcgt atagcataca ttatacgaag ttatctcgac gcggccgcgg
      301 atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattcggg tgtggggagg
      361 ttgtagcccg accctgcccc tccccccagg gaggttgaga gttctgggca gacggcagat
      421 gcataacaaa ggtgcatgat agctctgccc tgggggcaga gaagatggtt ggggaggggt
      481 ccctctcgtc ctagctcgag cttaatcact agtgaattcg ggtgtgggga ggttgtagcc
      541 cgaccctgcc cctcccccca gggaggttga gagttctggg cagacggcag atgcataaca
      601 aaggtgcatg atagctctgc cctgggggca gagaagatgg ttggggaggg gtccctctcg
      661 tcctagctcg agcttaatca ctagtgaatt cgggtgtggg gaggttgtag cccgaccctg
      721 cccctccccc cagggaggtt gagagttctg g
//
