LOCUS       sequence-153159-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-SH3 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pRS315e_pGal-dCas9-SH3
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        94..758
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    310..427
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     722..745
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 ttaaccctca ctaaagggaa caaaagctgg taccgggccc gaattctctc cttctcttag
       61 gtggcagagc aggtggaggg tcgaccatac tagtttcaaa aattcttact ttttttttgg
      121 atggacgcaa agaagtttaa taatcatatt acatggcatt accaccatat acatatccat
      181 atacatatcc atatctaatc ttacttatat gttgtggaaa tgtaaagagc cccattatct
      241 tagcctaaaa aaaccttctc tttggaactt tcagtaatac gcttaactgc tcattgctat
      301 attgaagtac ggattagaag ccgccgagcg ggtgacagcc ctccgaagga agactctcct
      361 ccgtgcgtcc tcgtcttcac cggtcgcgtt cctgaaacgc agatgtgcct cgcgccgcac
      421 tgctccgaac aataaagatt ctacaatact agcttttatg gttatgaaga ggaaaaattg
      481 gcagtaacct ggccccacaa accttcaaat gaacgaatca aattaacaac cataggatga
      541 taatgcgatt agttttttag ccttatttct ggggtaatta atcagcgaag cgatgatttt
      601 tgatctatta acagatatat aaatgcaaaa actgcataac cactttaact aatactttca
      661 acattttcgg tttgtattac ttcttattca aatgtaataa aagtatcaac aaaaaattgt
      721 taatatacct ctatacttta acgtcaagga gaaaaaaccc atggacaaga agtactccat
      781 tgggctcgct atcggcacaa acagcgtcgg ttgggccgtc attacggacg agtacaaggt
      841 gccg
//
