LOCUS       sequence-153160-         793 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-PmCDA1 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pRS315e_pGal-dCas9-PmCDA1
FEATURES             Location/Qualifiers
     source          1..793
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        17..35
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        112..776
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    328..445
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     740..763
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 attacgccaa gctcgaaatt aaccctcact aaagggaaca aaagctggta ccgggcccga
       61 attctctcct tctcttaggt ggcagagcag gtggagggtc gaccatacta gtttcaaaaa
      121 ttcttacttt ttttttggat ggacgcaaag aagtttaata atcatattac atggcattac
      181 caccatatac atatccatat acatatccat atctaatctt acttatatgt tgtggaaatg
      241 taaagagccc cattatctta gcctaaaaaa accttctctt tggaactttc agtaatacgc
      301 ttaactgctc attgctatat tgaagtacgg attagaagcc gccgagcggg tgacagccct
      361 ccgaaggaag actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag
      421 atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag cttttatggt
      481 tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatga acgaatcaaa
      541 ttaacaacca taggatgata atgcgattag ttttttagcc ttatttctgg ggtaattaat
      601 cagcgaagcg atgatttttg atctattaac agatatataa atgcaaaaac tgcataacca
      661 ctttaactaa tactttcaac attttcggtt tgtattactt cttattcaaa tgtaataaaa
      721 gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga aaaaacccat
      781 ggacaagaag tac
//
