LOCUS       sequence-153161-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  empty retroviral vector with IRES-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Kueh HY, Champhekar A, Nutt SL, Elowitz MB, Rothenberg EV
  TITLE     Positive feedback between PU.1 and the cell cycle controls myeloid 
            differentiation.
  JOURNAL   Science. 2013 Aug 9;341(6146):670-3. doi: 10.1126/science.1240831. 
            Epub 2013 Jul 18.
  PUBMED    23868921
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1240831"; journalName: "Science"; date: "2013-08-9-
            9"; volume: "341"; issue: "6146"; pages: "670-3"
COMMENT     Sequence Label: IRES-mCherry
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..21
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    89..656
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    106..658
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(250..267)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     477..496
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      679..688
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(830..848)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctccctttat ccagccctca ctccttctct aggcgccgga attagatctc tcgaggttaa
       61 cgaattctgc tatacgaagt tatccctctc cctccccccc ccctaacgtt actggccgaa
      121 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt
      181 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg
      241 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc
      301 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc
      361 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa
      421 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc
      481 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg
      541 gatctgatct ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg ttaaaaaaac
      601 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aagaacacga tgataatatg
      661 gccacaacca tgactagtgc caccatggtg agcaagggcg aggaggataa catggccatc
      721 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
      781 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      841 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
      901 ggctccaagg cctacgtgaa gcaccccgcc ga
//
