LOCUS       sequence-153162-        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged DmNot2 in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Haas G, Braun JE, Igreja C, Tritschler F, Nishihara T, Izaurralde E
  TITLE     HPat provides a link between deadenylation and decapping in metazoa.
  JOURNAL   J Cell Biol. 2010 Apr 19. 189(2):289-302.
  PUBMED    20404111
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2010 Apr 19. 189(2):289-302."
COMMENT     Sequence Label: pAc5.1B-EGFP-DmNot2
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..54
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(77..94)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(104..145)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 gatcctcgat cgaggctgat cagcgagctc aggccttaga aggcacagtc gaggctgatc
       61 agcgggttta aactcaatgg tgatggtgat gatgaccggt acgcgtagaa tcgagaccga
      121 ggagagggtt agggataggc ttaccttcga accgcgggcc ctctagatta tacagactgt
      181 ccattcataa acgcacttat attgggacat ttatctaatt tctcgggatc tatttggaaa
      241 accttggaca aacgtttcca actttgcgca tcaaagtagt agaaggtgcc gcgctctttt
      301 gtaccatttt tttcatattg atcaatgcca ggaattcgag ttatccatat tttctcctcc
      361 acgtggtatc gccactcgcg actatgcaat tcagcagctg ccatcaattg catcatatcg
      421 ccgatgttcg tgtaaaaaag gaaaaagagc aggtcttctt gcagcttttt cagtaccggc
      481 gctgttagtt tgtctcttat cgcaaagtta atcagatatt cgggtggcac gttgaactca
      541 acgtcttgtg ctctgcaggg ctgtgctacg aaggggccag cgaaggttgt atgcaagctc
      601 tcctgcgagt tcaggttcag gcctaaaccc gtgagatccg tacccaagga gagcgtcacc
      661 agattgggat ccgtttctgc agcccgaatg aacgtcagca ggccaaccat gccgaactgg
      721 ttgttcacca tggtcgctgg tatgtttgtg accttcccat ctggcgaagt ttgcacgccg
      781 ctctttacaa gtttatcgtt gctggaatta tcgttcagat gctcgccgcc cgctccgctc
      841 ccagacgagc cgcttccact ccccaggccg ccgcctacgc ccatcatggc attgctgttg
      901 ggtacgctgt tgacgccgcc aatgccgctg ttactgccca ccagtcctac atgggacccg
      961 ccgacaacgc cgctggctgc attgccgccg actcctccgc ctcctgagct accagctcct
     1021 ccg
//
