LOCUS       sequence-153163-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses nCas9(D10A)-PmCDA1 in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pRS315e_pGal-nCas9(D10A)-PmCDA1
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        18..36
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        353..794
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    353..470
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     765..788
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
ORIGIN
        1 gattacgcca agctcgaaat taaccctcac taaagggaac aaaagctggt accgggcccg
       61 aattctctcc ttctcttagg tggcagagca ggtggagggt cgaccatact agtttcaaaa
      121 attcttactt tttttttgga tggacgcaaa gaagtttaat aatcatatta catggcatta
      181 ccaccatata catatccata tacatatcca tatacatatc catatacata tccatatcta
      241 atcttactta tatgttgtgg aaatgtaaag agccccatta tcttagccta aaaaaacctt
      301 ctctttggaa ctttcagtaa tacgcttaac tgctcattgc tatattgaag tacggattag
      361 aagccgccga gcgggtgaca gccctccgaa ggaagactct cctccgtgcg tcctcgtctt
      421 caccggtcgc gttcctgaaa cgcagatgtg cctcgcgccg cactgctccg aacaataaag
      481 attctacaat actagctttt atggttatga agaggaaaaa ttggcagtaa cctggcccca
      541 caaaccttca aatgaacgaa tcaaattaac aaccatagga tgataatgcg attagttttt
      601 tagccttatt tctggggtaa ttaatcagcg aagcgatgat ttttgatcta ttaacagata
      661 tataaatgca aaaactgcat aaccacttta actaatactt tcaacatttt cggtttgtat
      721 tacttcttat tcaaatgtaa taaaagtatc aacaaaaaat tgttaatata cctctatact
      781 ttaacgtcaa ggagaaaaaa cccatggaca agaagtactc cattgggctc gctatcggca
      841 caaacagcgt
//
