LOCUS       sequence-153167-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses EGFP-tagged DmPAN2 in S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Braun JE, Huntzinger E, Fauser M, Izaurralde E
  TITLE     GW182 proteins directly recruit cytoplasmic deadenylase complexes to
            miRNA targets.
  JOURNAL   Mol Cell. 2011 Oct 7;44(1):120-33.
  PUBMED    21981923
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-10-7"; volume: "44(1)"; pages: "120-33"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmPAN2
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     38..55
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(78..95)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(105..146)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ggatcctcga tcgaggctga tcagcgagct caggccttag aaggcacagt cgaggctgat
       61 cagcgggttt aaactcaatg gtgatggtga tgatgaccgg tacgcgtaga atcgagaccg
      121 aggagagggt tagggatagg cttaccttcg aaccgcggtc agtcctctgg aaccttccac
      181 tgcagctgct ttccgcgttc gtaaagattc ttgagagcgt tggcaaactt cttctcctcc
      241 tgcagcttca aatagtgctt gtagagctgc agagtggtcc gtgcatcttc gatggagtcg
      301 tgcgtctcgg actgaatctt agtacccaag aaatgccagg ctagaaatct tagggaaacc
      361 atgcgatgat gtggcatgtg aaacaggtgc accgtatcta ttatctgctc cgacggtaca
      421 tagatgttta tgaccctaaa gtcgttcttc agaccatgtc cgacaaatat tactcctacg
      481 tcaacgaggt attttaattt ctggtatgag tacttcaagg cagtcagtcg ctttttgctg
      541 aagttagcgt ccaagtcgcc aggcttgatt cccgagaact gagttaggta gtccacgacc
      601 ttctcctggg tggatatgta atcgtccatg aatggaactc cttcagccgg gccttgtcct
      661 cgtatacatg aaatccgggc cacactcatg tggcatggct taatggtcgc cgttttgcca
      721 tccgggcgaa tctcattttc ctctggattt agggtaacga actcggcatc cattgcgacc
      781 agatcacccg actgcggcat ttcatctgac tgcaggggct gcagcgtggc gtctgcactt
      841 aagttgccta gcataggact cacaatttcc tctagaaaag ggttggatag atttgttggc
      901 gagccgctgc tgctctctga tggaatggtt tcttcacttt ccgtgacggt cgagctagta
      961 gtactagcag a
//
