LOCUS       sequence-153170-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        19..37
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(115..738)
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
ORIGIN
        1 tgattacgcc aagctcgaaa ttaaccctca ctaaagggaa caaaagctgg taccgggccc
       61 gaattctctc cttctcttag gtggcagagc aggtggaggg tcgaccatac tagaaacagc
      121 aggactctta gtggtgtgga gtatttttac ctgaatcata atggacaact cgctccgtcg
      181 tttttcagct cgcttcaaag tcttctcaag ccatctattc tcattcaatt gattgtgcga
      241 cgattggatg aatattttcc tgcaacattg gtagtgttca cttaccatta cattcaaccc
      301 aaccccgtta tctctgaggt tccacagccc aatttgattc ctcgcatttt tctcgtaata
      361 gagtttgcaa gcccagattt tcaaagtgtg gccgttcccc cgcagctcct ggttatacca
      421 ttctaagatc ttttcagcgc aatctgcaca aggactccag gatgagtacc aatttatcgt
      481 gaattgtccg gggttgtcgc gcaggtattc ttcgactttt ctaatgctaa agatttcggc
      541 gtgaatgcca cgttctgtcc cgctctgtgg tttattcaca gcatagcccc aaaaacacgc
      601 tctacgttca ccccgtcgtt ttaattcaaa gagaacgtag catctatgcg acacggattt
      661 tttgttgttg aaaaactgtt tcttaaacgt gtagatgtcc aacttctcat ggattctcac
      721 gtactcagcg tcggtcatcg atccgccgcc ttt
//
